TLR5 Antibody (19D759.2) - Azide and BSA Free

Name: TLR5 Antibody (19D759.2) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-80981
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-80981

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 19D759.2

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for TLR5 Antibody (19D759.2) - Azide and BSA Free

Western Blot: TLR5 Antibody (19D759.2)Azide and BSA Free [NBP2-80981]

Western Blot: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981] - BEAS-2B cells whole cell lysate. Image from verified customer review. Image from the standard format of this antibody.

Immunocytochemistry: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981]

Immunocytochemistry: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981] - Analysis of FFPE tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). Intense membrane staining of TLR5 was observed specifically in the pigmented basel cells of epidermal layer [10X Magnification] Image from

Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981]

Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981] - Human skin stained with TLR5 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Image from the standard format of this antibody.

Flow Cytometry: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981]

Flow Cytometry: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981] - Intracellular flow analysis of TLR5 in human monocytes using 0.5 ug of TLR5 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents TLR5 antibody. this antibody secondary antibody was used in this test. Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981]

Immunocytochemistry/Immunofluorescence: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981] - Staining using in-vitro cultured fixed non-permeabilized MCF7 breast cancer cells (ATCC). EpCAM (cell membrane) staining is represented in green (FITC filter), nuclei staining is represented in blue (DAPI filter). Image from a verified customer review using the Alexa Fluor 488 conjugated format of this antibody. Image from the standard format of this antibody.

Immunohistochemistry: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981]

Immunohistochemistry: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981] - Analysis of TLR5 in FFPE mouse brain tissue using TLR5 antibody at 5 ug/ml. Image from the standard format of this antibody.

Flow Cytometry: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981]

Flow Cytometry: TLR5 Antibody (19D759.2) - Azide and BSA Free [NBP2-80981] - Analysis using the PE conjugate of NBP2-24787. Staining of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs were stimulated 5 days with GM-CSF+IL4 prior to the staining. The cells were stained for surface markers CD14 and CD83 FITC, and TLR5 PE. CD14- cells were gated and stained with PE-conjugated isotype control (mouse IgG2a), and FITC-conjugated CD83 (left). CD14- cells were gated and stained with 1 ug PE-conjugated TLR5, and FITC-conjugated CD83 (right).

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Applications for TLR5 Antibody (19D759.2) - Azide and BSA Free

Application

Recommended Usage

Dot Blot

reported in scientific literature (PMID 27248820)

Flow (Cell Surface)

reported in scientific literature (PMID 24412598)

Flow (Intracellular)

reported in scientific literature (PMID 24412598)

Flow Cytometry

0.5-1 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

5 ug/ml. Use reported by customer review

Immunohistochemistry

1:10-1:500. Use reported in scientific literature (PMID 27392931)

Immunohistochemistry-Frozen

10 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-3 ug/ml

Application Notes

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TLR5

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Long Name

Toll-like Receptor 5

Alternate Names

TIL-3

Gene Symbol

TLR5

Additional TLR5 Products

Product Documents for TLR5 Antibody (19D759.2) - Azide and BSA Free

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Product Specific Notices for TLR5 Antibody (19D759.2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.