TLR5 Antibody (19D759.2) - BSA Free

Novus Biologicals | Catalog # NBP2-24787

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Cytometric Bead Assay Standard, Flow Cytometry Control, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 19D759.2

Format

BSA Free
View all TLR5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for TLR5 Antibody (19D759.2) - BSA Free

Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis of FFPE tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). Intense membrane staining of TLR5 was observed specifically in the pigmented basel cells of epidermal layer [10X Magnification]
Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis using the PE conjugate of NBP2-24787. Staining of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs were stimulated 5 days with GM-CSF+IL4 prior to the staining. The cells were stained for surface markers CD14 and CD83 FITC, and TLR5 PE. CD14- cells were gated and stained with PE-conjugated isotype control (mouse IgG2a), and FITC-conjugated CD83 (left). CD14- cells were gated and stained with 1 ug PE-conjugated TLR5, and FITC-conjugated CD83 (right).
Western Blot: TLR5 Antibody (19D759.2)BSA Free [NBP2-24787]

Western Blot: TLR5 Antibody (19D759.2)BSA Free [NBP2-24787]

Western Blot: TLR5 Antibody (19D759.2) [NBP2-24787] - BEAS-2B cells whole cell lysate. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Immunocytochemistry/ Immunofluorescence: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Immunocytochemistry/Immunofluorescence: TLR5 Antibody (19D759.2) [NBP2-24787] - Staining using in-vitro cultured fixed non-permeabilized MCF7 breast cancer cells (ATCC). EpCAM (cell membrane) staining is represented in green (FITC filter), nuclei staining is represented in blue (DAPI filter). Image from a verified customer review using the Alexa Fluor 488 conjugated format of this antibody.
Immunohistochemistry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Immunohistochemistry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

TLR5-Antibody-19D759-2-Immunohistochemistry-NBP2-24787-img0013.jpg
Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787] - Flow analysis of TLR5 in mouse MC38 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Human skin stained with TLR5 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.
Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) [NBP2-24787] - Analysis of TLR5 in FFPE mouse brain tissue using TLR5 antibody at 5 ug/ml.
Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Flow Cytometry: TLR5 Antibody (19D759.2) - BSA Free [NBP2-24787]

Flow Cytometry: TLR5 Antibody (19D759.2) [NBP2-24787] - Intracellular flow analysis of TLR5 in human monocytes using 0.5 ug of TLR5 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents TLR5 antibody. this antibody secondary antibody was used in this test.

Applications for TLR5 Antibody (19D759.2) - BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature (PMID 27248820)

Flow (Cell Surface)

reported in scientific literature (PMID 24412598)

Flow (Intracellular)

0.5-1 ug/10^6 cells. Use reported in scientific literature (PMID 24412598)

Flow Cytometry

0.5-1 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

5 ug/ml. Use reported by customer review

Immunohistochemistry

1:10-1:500. Use reported in scientific literature (PMID 27392931)

Immunohistochemistry-Frozen

10 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-3 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reviewed Applications

Read 1 review rated 4 using NBP2-24787 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TLR5

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Long Name

Toll-like Receptor 5

Alternate Names

TIL-3

Gene Symbol

TLR5

Additional TLR5 Products

Product Documents for TLR5 Antibody (19D759.2) - BSA Free

Certificate of Analysis

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Product Specific Notices for TLR5 Antibody (19D759.2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TLR5 Antibody (19D759.2) - BSA Free

Customer Reviews for TLR5 Antibody (19D759.2) - BSA Free (1)

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Customer Images

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  • TLR5 Antibody (19D759.2)
    Name: Monica Sauter
    Application: Immunohistochemistry-Paraffin
    Sample Tested: formalin fixed paraffin embedded macaque retina tissue
    Species: maccaca mulatta
    Verified Customer | Posted 04/06/2018
    TLR5 antibody stains retinal ganglion cells and round cells in the inner nuclear layer of paraffin embedded rhesus macaque retina tissue
    heat mediated antigen retrieval (pH 6 citrate buffer) 1:50 dilution of antibody, overnight at 4C goat anti-mouse AlexaFluor 488 secondary antibody (1:400, 1 hr RT)
    TLR5 Antibody (19D759.2) - BSA Free NBP2-24787

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