Transgelin/TAGLN/SM22 alpha Antibody

Novus Biologicals | Catalog # NB600-507

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Knockdown Validated

Cited:

Western Blot, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Transgelin/TAGLN Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-MTGYGRPRQIIS corresponding to C-Terminus according to NP_003177.2.

Reactivity Notes

This antibody has been successfully used in Western blot in Rat (PMID: 27531064). Use in Mouse reported in scientific literature (PMID: 32134397).

Specificity

NP_003177.2 and NP_001001522.1 represent identical protein.The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumor suppressor.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Transgelin/TAGLN/SM22 alpha Antibody

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of Human Testes lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507]

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507]

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of paraffin embedded Human Prostate. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507]

Transgelin-TAGLN-SM22-alpha-Antibody-Western-Blot-NB600-507-img0002.jpg

Applications for Transgelin/TAGLN/SM22 alpha Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

1:4000

Western Blot

0.1 - 1 ug/mL
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 31965901). Knockdown validation (PMID: 32134397).

Reviewed Applications

Read 1 review rated 5 using NB600-507 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Transgelin/TAGLN

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Alternate Names

SM22 alpha, TAGLN, WS3-10

Entrez Gene IDs

6876 (Human); 21345 (Mouse); 25123 (Rat)

Gene Symbol

TAGLN

UniProt

Q01995, NP_003177.2 (Human)

Additional Transgelin/TAGLN Products

Product Documents for Transgelin/TAGLN/SM22 alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Transgelin/TAGLN/SM22 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Transgelin/TAGLN/SM22 alpha Antibody

Customer Reviews for Transgelin/TAGLN/SM22 alpha Antibody (1)

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Customer Images

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  • Transgelin/TAGLN/SM22 alpha Antibody
    Name: Katie Tobik
    Application: Immunofluorescence
    Sample Tested: adipose derived stem cells differentiated to smooth muscle cells
    Species: Human
    Verified Customer | Posted 11/03/2017
    Transgelin/TAGLN/SM22 alpha Antibody NB600-507

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Protocols

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FAQs for Transgelin/TAGLN/SM22 alpha Antibody

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  • Q: We are using the Transgelin antibody (NB600-507) for western analysis of mouse and human uterine tissue. In the mouse we see a single band of 22 kDa but in the human we see a band of 12 kDa. Any thoughts on this? I can not find any information on a smaller transgelin band.

    A: I have searched for an explanation for the lower molecular weight band you are detecting, and have found that this paper, PMC2789179 says: 'It should be noted that the expression analysis of TAGLN is complicated by the fact that multiple bands were detected on the Western blots. Similar observations have been described and attributed to C-terminal proteolysis. While the sum of band intensities was used for the analyses here, it is possible that TAGLN regulation is not a simple change in quantity of one defined species, but may, in fact, be subject to posttranslationally regulated events such as modification or degradation.'

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