Triosephosphate isomerase Antibody

Novus Biologicals | Catalog # NBP1-31470

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Bovine

Predicted:

Canine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Triosephosphate isomerase Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 29892502).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Triosephosphate isomerase Antibody

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Triosephosphate-isomerase-Antibody-Western-Blot-NBP1-31470-img0022.jpg
Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody [NBP1-31470]

Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody [NBP1-31470]

Immunocytochemistry/Immunofluorescence: Triosephosphate isomerase Antibody [NBP1-31470] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase stained by Triosephosphate isomerase antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.
Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NBP1-31470]

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NBP1-31470]

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NBP1-31470] - Paraffin-embedded SNU-16 xenograft, using antibody at 1:100 dilution.
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470]

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Triosephosphate isomerase Antibody

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] -

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat & human cells. a-d Immunodetection of Eno1, Tpi1, Pgam1 & beta 2-Tub (load control) in Poly I:C transfected & Control cells at 4, 8 & 20 hpt (a: PaKiT03, b: PaLuT02, c: HEK293T, d: HeLa). e-h Densitometry analysis following immunodetection of Eno1, Tpi1, Pgam1. Average ratios were calculated as Poly I:C/Control normalised to beta -Tub. Error bars represent the standard error of the mean from two technical replicates (e: PaKiT03, f: PaLuT02, g: HEK293T, h: HeLa) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26535029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Triosephosphate isomerase Antibody

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] -

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat & human cells. a-d Immunodetection of Eno1, Tpi1, Pgam1 & beta 2-Tub (load control) in Poly I:C transfected & Control cells at 4, 8 & 20 hpt (a: PaKiT03, b: PaLuT02, c: HEK293T, d: HeLa). e-h Densitometry analysis following immunodetection of Eno1, Tpi1, Pgam1. Average ratios were calculated as Poly I:C/Control normalised to beta -Tub. Error bars represent the standard error of the mean from two technical replicates (e: PaKiT03, f: PaLuT02, g: HEK293T, h: HeLa) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26535029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Triosephosphate isomerase Antibody

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] -

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat & human cells. a-d Immunodetection of Eno1, Tpi1, Pgam1 & beta 2-Tub (load control) in Poly I:C transfected & Control cells at 4, 8 & 20 hpt (a: PaKiT03, b: PaLuT02, c: HEK293T, d: HeLa). e-h Densitometry analysis following immunodetection of Eno1, Tpi1, Pgam1. Average ratios were calculated as Poly I:C/Control normalised to beta -Tub. Error bars represent the standard error of the mean from two technical replicates (e: PaKiT03, f: PaLuT02, g: HEK293T, h: HeLa) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26535029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Triosephosphate isomerase Antibody

Application
Recommended Usage

ELISA

Assay dependent

Flow Cytometry

Reported in scientific literature (PMID: 22262862)

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-31470 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Triosephosphate isomerase

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Alternate Names

EC 5.3.1.1, MGC88108, TIM, TPI, triosephosphate isomerase, Triose-phosphate isomerase, triosephosphate isomerase 1

Entrez Gene IDs

7167 (Human)

Gene Symbol

TPI1

UniProt

P60174 (Human)

Additional Triosephosphate isomerase Products

Product Documents for Triosephosphate isomerase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Triosephosphate isomerase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Triosephosphate isomerase Antibody

Customer Reviews for Triosephosphate isomerase Antibody (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Immortalised Pteropus alecto kidney cell lysate (PaKiT03)
    Species: Other
    Verified Customer | Posted 09/21/2015
    WB analysis of Triosephosphate isomerase in Pteropus alecto
    Triosephosphate isomerase Antibody NBP1-31470

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