TrkB [p Tyr816] Antibody - Azide and BSA Free

Western Blot: TrkB [p Tyr816] Antibody - Azide and BSA Free [NBP1-03499] -

BDNF recombinant protein intervention increased phosphorylation of TrkB. (A-B) The quantification of the Western blots results and a representative Western blot showing BDNF and p-TrkB protein isolated from the ipsilateral SCDH 48 h after 2nd injection. (C) Representative immunofluorescence images of BDNF (red) merged with p-TrkB (green) in ipsilateral L4-6 SCDH of BDNF recombinant protein intervention rats. (D-F) The immunofluorescence quantification of BDNF and p-TrkB co-expression cells in ipsilateral L4-6 SCDH. (G-H) Evaluation of BDNF and p-TrkB gene expression in ipsilateral L4-6 SCDH using qPCR in the HP + PBS, HP + EA + PBS, HP + EA + BDNF groups 48 h after 2nd injection. Data are presented as mean +/- SEM, n = 5; *p < 0.05, **p < 0.01 vs HP + PBS group, #p < 0.05, ##p < 0.01 vs HP + EA + PBS group. Two-way ANOVA with Tukey's post-hoc test was applied in. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36875547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TrkB [p Tyr816] Antibody - Azide and BSA Free [NBP1-03499] -

The expression of BDNF-TrkB signaling in the SDH of CYP-induced cystitis. a Changes of the mechanical threshold in CYP-induced cystitis model. Compared to that in the control group, the mechanical threshold of the cystitis group decreased significantly after the CYP injection and remained low until day 17, and the minimum threshold value was reached on day 12. The expression of b BDNF, c TrkB, and dp-TrkB were evaluated by western blots. Compared to the control group, they were upregulated on days 8, 12, and 17. e Immunofluorescence double staining assay of BDNF and p-TrkB in the SDH. BDNF and p-TrkB (red), NeuN, GFAP, and OX-42( green), co-localization (yellow). BDNF was mainly colocalized in neurons which mainly located in Laminate II to IV. And TrkB receptors expressed in neurons, microglia, and astrocytes. The white dotted lines in picture “BDNF/NeuN" showed the laminate of the SDH according to Rexed and Steiner. Scale bar = 100 μm. All data were calculated as mean +/- SEM (n = 5 per group). *p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TrkB [p Tyr816] Antibody - Azide and BSA Free [NBP1-03499] -

The effect of GSB-106 on the BDNF content and the site-specific phosphorylation of TrkB receptor in the prefrontal cortex and the hippocampus of mice subjected to UCMS. (a) BDNF, (b) pTrkB(Tyr706/707), (c) pTrkB(Tyr816), (d) pTrkB(Tyr515) proteins expression in mice prefrontal cortex and hippocampus. After the completion of UCMS procedure and the compound treatment, mice were decapitated and brain structures were collected. Protein extracts were subjected to polyacrylamide gel electrophoresis and transferred for Western blotting. Blots were probed with anti-BDNF, anti-phosphorylated TrkB antibodies and then reprobed with anti-TrkB antibody, anti-a-tubulin antibodies. For every picture, representative images for immunoblots are shown on the top panels, and quantitative data are shown on the bottom panels (red dots on the graphs correspond to mice 4, 8, 10 and 15; dark dots are all mice samples in the group). All data are expressed as mean +/- SEM (n = 3; the significance of intergroup differences was estimated by one-way analysis of the variance (ANOVA), followed by Tukey’s multiple comparisons test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34948177), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TrkB [p Tyr816] Antibody - Azide and BSA Free [NBP1-03499] -

BDNF and TrkB in the L4-L6 SCDH plays a key role in the hyperalgesic priming model. (A-B) The quantification of the Western blots results and a representative Western blot showing BDNF and p-TrkB protein isolated from the ipsilateral SCDH 48 h after 2nd injection. (C) Representative immunofluorescence images of BDNF (red) merged with p-TrkB (green) in ipsilateral L4-6 SCDH of hyperalgesic priming rats. (D-F) The immunofluorescence quantification of BDNF and p-TrkB co-expression cells in ipsilateral L4-6 SCDH. (G-H) Evaluation of BDNF and p-TrkB gene expression in ipsilateral L4-6 SCDH using qPCR in the sHP, HP, HP + EA, HP + sEA groups 48 h after 2nd injection. Data are presented as mean +/- SEM, n = 5; *p < 0.05, **p < 0.01 vs sHP group, #p < 0.05, ##p < 0.01 vs HP group. Two-way ANOVA with Tukey's post-hoc test was applied in. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36875547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TrkB [p Tyr816] Antibody - Azide and BSA Free [NBP1-03499] -

BDNF recombinant protein intervention increased phosphorylation of TrkB. (A-B) The quantification of the Western blots results and a representative Western blot showing BDNF and p-TrkB protein isolated from the ipsilateral SCDH 48 h after 2nd injection. (C) Representative immunofluorescence images of BDNF (red) merged with p-TrkB (green) in ipsilateral L4-6 SCDH of BDNF recombinant protein intervention rats. (D-F) The immunofluorescence quantification of BDNF and p-TrkB co-expression cells in ipsilateral L4-6 SCDH. (G-H) Evaluation of BDNF and p-TrkB gene expression in ipsilateral L4-6 SCDH using qPCR in the HP + PBS, HP + EA + PBS, HP + EA + BDNF groups 48 h after 2nd injection. Data are presented as mean +/- SEM, n = 5; *p < 0.05, **p < 0.01 vs HP + PBS group, #p < 0.05, ##p < 0.01 vs HP + EA + PBS group. Two-way ANOVA with Tukey's post-hoc test was applied in. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36875547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.