TSLP Antibody - BSA Free

Novus Biologicals | Catalog # NB110-55234

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Tagged Protein Expression, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TSLP Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 19 amino acids near the center of human TSLP. The immunogen is located within the amino acids 40-90 of TSLP. Amino Acid Sequence: SGTKSTEFNNTVSCSNRPH

Specificity

Human TSLP has 2 isoforms, including isoform 1 (159aa, 18kD) and isoform 2 (63aa, 7kD). Mouse TSLP has one isoform (140aa, 16kD) and Rat TSLP also has one isoform (135aa, 16kD). NB110-55234 can detect only longer human isoform, but cannot detect shorter human isoform, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TSLP Antibody - BSA Free (NB110-55234) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-TSLP Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TSLP Antibody - BSA Free

Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP AntibodyBSA Free [NB110-55234]

TSLP-Antibody-Western-Blot-NB110-55234-img0013.jpg
Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55234]

Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55234]

TSLP-Antibody-Immunohistochemistry-NB110-55234-img0012.jpg
Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP Antibody [NB110-55234] - Total protein levels of TSLP in patients with AERD and subjects with CRS measured by using Western blotting of 3 representative samples per group. This image was submitted via customer Review.
Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation in Human Heart (Lane 1) and Human Prostate (Lane 2).Loading: 15 ug of lysates per lane. Antibodies: TSLP NB110-55234 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP AntibodyBSA Free [NB110-55234]

Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation of TSLP with Human Recombinant protein. Loading: 30ng of TSLP partial human recombinant protein per lane. Antibodies: TSLP NB110-55234 (Lane 1: 0.25 ug/mL, Lane 2: 0.5 ug/mL, Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
TSLP Antibody - BSA Free

Western Blot: TSLP Antibody - BSA Free [NB110-55234] -

Western Blot: TSLP Antibody - BSA Free [NB110-55234] - Validation in Mouse A-20 Cell Line. Loading: 15 ug of lysates per lane.Antibodies: TSLP (A: 0.5 ug/mL, B: 1 ug/mL, C: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
TSLP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TSLP Antibody - BSA Free [NB110-55234] -

Immunocytochemistry/ Immunofluorescence: TSLP Antibody - BSA Free [NB110-55234] - Validation of TSLP in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling TSLP with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
TSLP Antibody - BSA Free

Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55234] -

Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55234] - Validation of TSLP in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-TSLP antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for TSLP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.25-4 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB110-55234 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TSLP

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse)

Gene Symbol

TSLP

UniProt

Q969D9

Additional TSLP Products

Product Documents for TSLP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TSLP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TSLP Antibody - BSA Free

Customer Reviews for TSLP Antibody - BSA Free (1)

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  • TSLP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Nasal tissue
    Species: Human
    Verified Customer | Posted 07/01/2017
    Total protein levels of TSLP in patients with AERD and subjects with CRS measured by using Western blotting of 3 representative samples per group.
    TSLP Antibody - BSA Free NB110-55234

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