TUFM Antibody (CL2242) - BSA Free

Novus Biologicals | Catalog # NBP2-36751

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Predicted:

Mouse (95%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2242

Format

BSA Free
View all TUFM Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P49411, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TUFM Antibody (CL2242) - BSA Free

Western Blot: TUFM Antibody (CL2242) [NBP2-36751]

Western Blot: TUFM Antibody (CL2242) [NBP2-36751]

Western Blot: TUFM Antibody (CL2242) [NBP2-36751] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TUFM antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751] - Staining in MCF7 cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.
Western Blot: TUFM Antibody (CL2242) [NBP2-36751]

Western Blot: TUFM Antibody (CL2242) [NBP2-36751]

Western Blot: TUFM Antibody (CL2242) [NBP2-36751] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
Western Blot: TUFM Antibody (CL2242) [NBP2-36751]

Western Blot: TUFM Antibody (CL2242) [NBP2-36751]

Western Blot: TUFM Antibody (CL2242) [NBP2-36751] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction, Lane 3: Human cell line HeLa membrane fraction, Lane 4: Human cell line HeLa nuclear fraction, Lane 5: Human cell line HeLa chromatin fraction, Lane 6: Human cell line HeLa cytos
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751] - Staining in HeLa cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751] - Staining in U2OS cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751] - Staining in U251 cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2242) [NBP2-36751] - Staining in A431 cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751] - Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2242) [NBP2-36751] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate staining in glomerular cells.

Applications for TUFM Antibody (CL2242) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TUFM

TUFM encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Alternate Names

EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial

Gene Symbol

TUFM

UniProt

P49411 (Human)

Additional TUFM Products

Product Documents for TUFM Antibody (CL2242) - BSA Free

Certificate of Analysis

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Product Specific Notices for TUFM Antibody (CL2242) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TUFM Antibody (CL2242) - BSA Free

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Protocols

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