VEGFR1/Flt-1 Antibody (EWC) [Biotin]

Name: VEGFR1/Flt-1 Antibody (EWC) [Biotin]
Brand: Novus Biologicals
SKU: NBP1-18636
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-18636

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # EWC

View all VEGFR1/Flt-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human soluble extracellular Flt-1 (D5)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Type I membrane protein (Flt1) and soluble (SFlt1).

Marker

Endothelial Cell Marker

Specificity

The monoclonal will detect native and denaturated human VEGFR-1/ Flt-1 in ELISA experiments and on the surface of different human cell types. The will also detect mouse Flt-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for VEGFR1/Flt-1 Antibody (EWC) [Biotin]

Application

Recommended Usage

ELISA

2.5 ug/ml

Immunoprecipitation

1-5 ug/ml

Western Blot

2-5 ug/ml

Application Notes

This is useful for the following applications: ELISA, Western blotting, immunofluorescence, and immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Formulation

PBS; 50X BSA

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VEGFR1/Flt-1

VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody (EWC) [Biotin]

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Certificate of Analysis

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Product Specific Notices for VEGFR1/Flt-1 Antibody (EWC) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.