VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free

Name: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-81008
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-81008

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 1B6

Format

Azide and BSA Free

View all VEGFR2/KDR/Flk-1 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human VEGFR2 protein (between amino acids 300-600) [Uniprot: P35968]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free

Western Blot: VEGFR2/KDR/Flk-1 Antibody (1B6)Azide and BSA Free [NBP2-81008]

Western Blot: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008] - Analysis of VEGF R2/KDR/Flk-1 (1B6) in purified human recombinant protein. Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008]

Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008] - Detection in HUVEC cells at 1:50 Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008]

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008] - Analysis of FFPE transverse section of normal human skeletal muscle with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm. Image from the standa

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008]

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008] - HepG2 cells were stained (surface) with VEGF R2/KDR/Flk-1 antibody (clone: 2B5.1B6, orange) or isotype control (mouse IgG1, blue) and positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008]

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm. Image f

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008]

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008] - Analysis of FFPE tissue section of human liver cancer with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cancer cells showed a specific and strong staining in the cytoplasm. Image from the standard format of this an

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008]

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free [NBP2-81008] - Analysis of FFPE tissue section of human normal kidney with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of prote

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Applications for VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR2/KDR/Flk-1

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Gene Symbol

KDR

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for VEGFR2/KDR/Flk-1 Antibody (1B6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.