VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free

Name: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-81009
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-81009

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 2C6

Format

Azide and BSA Free

View all VEGFR2/KDR/Flk-1 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human VEGFR2 protein (between amino acids 300-600) [UniProt# P35968].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free

Western Blot: VEGFR2/KDR/Flk-1 Antibody (2C6)Azide and BSA Free [NBP2-81009]

Western Blot: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein. Image from the standard format of this antibody.

Immunocytochemistry: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009]

Immunocytochemistry: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of FFPE tissue section of Human Hepatocellular Carcinoma with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009]

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of FFPE tissue section of Human SCC of Esophagus with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm while the tumor stroma was negative for VEGF R2/KDR/Flk-1 immunopositivity. Image f

Flow (Cell Surface): VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009]

Flow (Cell Surface): VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - HepG2 cells were stained (surface) with antibody (orange) or isotype control, mouse IgG1 (blue). Positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009]

Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Antibody (1:50) was tested in HUVEC cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.

Immunohistochemistry: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009]

Immunohistochemistry: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Aanalysis of FFPE transverse section of normal human skeletal muscle with antibody at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm. Image from the standard format of this antibody.

Immunohistochemistry: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009]

Immunohistochemistry: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009]

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of FFPE tissue section of human normal kidney with antibody at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of VEGF R2/KDR/Flk-1 protein. Image from the standard f

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Applications for VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1.0 ug/ml

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR2/KDR/Flk-1

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Gene Symbol

KDR

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free

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Product Specific Notices for VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.