VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free

Name: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP1-18644
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-18644

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 4H3

Format

Azide and BSA Free

View all VEGFR2/KDR/Flk-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human soluble extracellular KDR (D7)

Marker

Endothelial Cell Marker

Specificity

The monoclonal antibody will detect native human VEGFR-2/KDR on the surface of different human cell types.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Clone #3 changed to 4H3.

Scientific Data Images for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]

Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644] - VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - IF double staining of human KDR in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with (A) mouse anti-human KDR, (B) rabbit antihuman KDR (C) DAPI and (D) merged. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour (1:600) and goat anti-mouse PE.

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Analysis using the Biotin conjugate of NBP1-18644. Staining of VEGFR-2/KDR expression in HUVE cells.

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]

Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Fig. 1: Flow analysis of VEGF R2/KDR/Flk-1 expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC).

ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]

ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - 1 Sandwich-ELISA using soluble KDR (D7) as standard. Mouse anti-human VEGFR-2 was used as capture antibody, Biotinylated rabbit anti-human VEGFR-2 was used for detection.

ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]

ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Total lysate from PAE/KDR cells was measured for expression of VEGFR- 1/KDR by a standard Sandwich ELISA using a mouse monoclonal anti-human VEGFR-1 antibody for capturing and a Biotin conjugated rabbit polyclonal anti-human VEGFR-1 antibody for detection. Total lysate from PAE/Flt-1 and PAE/FLT-4 was used as negative control. As positive control, total lysate from PAE/Flt-1 was spiked with recombinant human sKDR.

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Applications for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free

Application

Recommended Usage

ELISA

1-5 ug/ml

Flow Cytometry

2 - 5 ug/ml

Immunocytochemistry/ Immunofluorescence

1-5 ug/ml

Western Blot

1-2 ug/ml

Application Notes

This antibody is Cytof ready.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VEGFR2/KDR/Flk-1

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Entrez Gene IDs

3791 (Human)

Gene Symbol

KDR

UniProt

P35968

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free

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Product Specific Notices for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.