Vimentin Antibody (CL0157) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43924

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0157

Format

Azide and BSA Free
View all Vimentin Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P08670, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Vimentin Antibody (CL0157) - Azide and BSA Free

Vimentin Antibody (CL0157) - Azide and BSA Free Western Blot: Vimentin Antibody [NBP3-43924]

Western Blot: Vimentin Antibody [NBP3-43924]

Lane 1: Marker [kDa]
Lane 2: Human cell line HeLa cytoplasmic fraction
Lane 3: Human cell line HeLa membrane fraction
Lane 4: Human cell line HeLa nuclear fraction
Lane 5: Human cell line HeLa chromatin fraction
Lane 6: Human cell line HeLa cytoskeletal fraction
Vimentin Antibody (CL0157) - Azide and BSA Free Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Staining of human cerebellum shows immunoreactivity in the endothelial cells.
Vimentin Antibody (CL0157) - Azide and BSA Free Western Blot: Vimentin Antibody [NBP3-43924]

Western Blot: Vimentin Antibody [NBP3-43924]

Lane 1: Marker [kDa]
Lane 2: Human tonsil tissue lysate
Vimentin Antibody (CL0157) - Azide and BSA Free Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.
Vimentin Antibody (CL0157) - Azide and BSA Free Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Staining of human prostate shows strong immunoreactivity in the connective tissue.
Vimentin Antibody (CL0157) - Azide and BSA Free Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Staining of human skin shows distinct positivity in fibroblasts.
Vimentin Antibody (CL0157) - Azide and BSA Free Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-43924]

Staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.
Vimentin Antibody (CL0157) - Azide and BSA Free Western Blot: Vimentin Antibody [NBP3-43924]

Western Blot: Vimentin Antibody [NBP3-43924]

Analysis in human cell lines U-251MG and MCF-7 using Anti-Vimentin antibody. Corresponding Vimentin RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Applications for Vimentin Antibody (CL0157) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Vimentin

Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

Alternate Names

VIM

Gene Symbol

VIM

Additional Vimentin Products

Product Documents for Vimentin Antibody (CL0157) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Vimentin Antibody (CL0157) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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