Viral wnvNS3 Protease Antibody

Catalog # Availability Size / Price Qty
Detection of wnvNS3 Protease by Western Blot.
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Product Details
Citations (6)
Supplemental Products

Viral wnvNS3 Protease Antibody Summary

Species Reactivity
Detects West Nile Virus NS3 Protease in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant wnvNS3 Protease, expressed as a fusion protein with cofactor NS2L
Accession # ABD85079
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Viral wnvNS3 Protease (Catalog # 2907‑SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of wnvNS3 Protease antibody by Western Blot. View Larger

Detection of wnvNS3 Protease by Western Blot. Western blot shows Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Viral wnvNS3 Protease Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. The purified recombinant protein has autocatalytic activity that can lead to protein degradation. It is therefore important to store the sample below -20° C and to keep on ice while working with the sample.

  1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
  2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.
Long Name
West Nile Virus NS3 Protease
Entrez Gene IDs
912267 (Viral)
Alternate Names
wnvNS3 Protease

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Citations for Viral wnvNS3 Protease Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. TRIM5? Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation
    Authors: AI Chiramel, NR Meyerson, KL McNally, RM Broeckel, VR Montoya, O Méndez-Sol, SJ Robertson, GL Sturdevant, KJ Lubick, V Nair, BH Youseff, RM Ireland, CM Bosio, K Kim, J Luban, VM Hirsch, RT Taylor, F Bouamr, SL Sawyer, SM Best
    Cell Rep, 2019;27(11):3269-3283.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Interleukin-1? Signaling in Dendritic Cells Induces Antiviral Interferon Responses
    Authors: LD Aarreberg, C Wilkins, HJ Ramos, R Green, MA Davis, K Chow, M Gale
    MBio, 2018;9(2):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Arsenite-induced stress granule formation is inhibited by elevated levels of reduced glutathione in West Nile virus-infected cells
    Authors: M Basu, SC Courtney, MA Brinton
    PLoS Pathog, 2017;13(2):e1006240.
    Species: Hamster
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. IRF-3, IRF-5, and IRF-7 coordinately regulate the type I IFN response in myeloid dendritic cells downstream of MAVS signaling.
    Authors: Lazear H, Lancaster A, Wilkins C, Suthar M, Huang A, Vick S, Clepper L, Thackray L, Brassil M, Virgin H, Nikolich-Zugich J, Moses A, Gale M, Fruh K, Diamond M
    PLoS Pathog, 2013;9(1):e1003118.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Identification of novel host cell binding partners of Oas1b, the protein conferring resistance to flavivirus-induced disease in mice.
    Oncogene, 2012;86(15):7953-63.
    Species: Virus
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.
    Authors: Wang P, Arjona A, Zhang Y
    Nat. Immunol., 2010;11(10):912-9.
    Species: Virus
    Sample Types: Cell Lysates
    Applications: Western Blot


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Secondary Antibodies

Goat IgG (H+L) Antibody


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