Wnt-3a Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-74183
![Western Blot: Wnt-3a Antibody [NBP1-74183]](https://resources.rndsystems.com/images/products/Wnt-3a-Antibody-Western-Blot-NBP1-74183-img0009.jpg "Western Blot: Wnt-3a Antibody [NBP1-74183]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
Synthetic peptides corresponding to the C terminal of Wnt3a. Immunizing peptide sequence IGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEA. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for Wnt-3a Antibody - BSA Free
Western Blot: Wnt-3a Antibody [NBP1-74183]
Western Blot: Wnt-3a Antibody [NBP1-74183] - Sample Tissue: Mouse Heart, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane, Gel Concentration: 0.12%![Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-74183]](https://resources.rndsystems.com/images/products/Wnt-3a-Antibody-Immunohistochemistry-Paraffin-NBP1-74183-img0012.jpg "Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-74183]")
Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-74183]
Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-74183] - Human placenta tissue at an antibody concentration of Dilution: 1:500.![Western Blot: Wnt-3a Antibody [NBP1-74183]](https://resources.rndsystems.com/images/products/Wnt-3a-Antibody-Western-Blot-NBP1-74183-img0011.jpg "Western Blot: Wnt-3a Antibody [NBP1-74183]")
Western Blot: Wnt-3a Antibody [NBP1-74183]
Western Blot: Wnt-3a Antibody [NBP1-74183] - Titration: 1 ug/ml Positive Control: Mouse Heart lysate.![Western Blot: Wnt-3a Antibody [NBP1-74183]](https://resources.rndsystems.com/images/products/Wnt-3a-Antibody-Western-Blot-NBP1-74183-img0010.jpg "Western Blot: Wnt-3a Antibody [NBP1-74183]")
Western Blot: Wnt-3a Antibody [NBP1-74183]
Western Blot: Wnt-3a Antibody [NBP1-74183] - Mouse Heart. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide.Applications for Wnt-3a Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Wnt-3a
Long Name
Wingless-type MMTV Integration Site Family, Member 3a
Alternate Names
Wnt3a
Gene Symbol
WNT3A
UniProt
Additional Wnt-3a Products
Product Documents for Wnt-3a Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Wnt-3a Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Wnt-3a Antibody - BSA Free
Powered by Bioz
Customer Reviews for Wnt-3a Antibody - BSA Free
There are currently no reviews for this product. Be the first to review Wnt-3a Antibody - BSA Free and earn rewards!
Have you used Wnt-3a Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
