Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Monkey

Cited:

Human, Mouse

Predicted:

Amphibian (90%), Guinea Pig (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Wnt-5a Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 190-230 of human Wnt-5a was used as the immunogen for this antibody, GenBank no NP_003383.2.

Reactivity Notes

The amino acid sequence used as immunogen is 90% homologous in newt (Pleurodeles waltl), and 88% in Xenopus and axolotl (Ambystoma mexicanum). Reactivity to Petarus breviceps reported in verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Wnt-5a Antibody - BSA Free

Western Blot: Wnt-5a AntibodyBSA Free [NBP2-24752]

Western Blot: Wnt-5a AntibodyBSA Free [NBP2-24752]

Western Blot: Wnt-5a Antibody [NBP2-24752] - Analysis of Wnt-5a in human heart lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat heart
Immunohistochemistry-Frozen: Wnt-5a Antibody - BSA Free [NBP2-24752]

Immunohistochemistry-Frozen: Wnt-5a Antibody - BSA Free [NBP2-24752]

Immunohistochemistry-Frozen: Wnt-5a Antibody [NBP2-24752] - P. breviceps epithelial expression of Wnt5a at 1:100 dilution. Tissue samples Cryo-section fixed (12um slices) in OCT, washed for 10min in PBT (PBS + 0.1% tween), blocked for 1hr in 3%BSA/PBS, and then incubated with the antibody overnight at 4C. The next day washed 3x for 10min in PBT and incubated for one hr in Alexa 488 (1:500), washed 3x10min in PBS, and coverslipped. Image from verified customer review.
Immunohistochemistry-Paraffin: Wnt-5a Antibody - BSA Free [NBP2-24752]

Immunohistochemistry-Paraffin: Wnt-5a Antibody - BSA Free [NBP2-24752]

Immunohistochemistry-Paraffin: Wnt-5a Antibody [NBP2-24752] - Analysis of Wnt-5a in FFPE human breast tumor using an isotype control (left) and Wnt-5a antibody (right)
Wnt-5a Antibody - BSA Free Immunohistochemistry-Paraffin: Wnt-5a Antibody - BSA Free [NBP2-24752]

Immunohistochemistry-Paraffin: Wnt-5a Antibody - BSA Free [NBP2-24752]

Analysis of a FFPE tissue section of human placenta using 1:200 dilution of Wnt-5a antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Applications for Wnt-5a Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

reported by customer review

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

0.5-2 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP2-24752 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-5a

Wnt5A is a secreted protein found in the extracellular region that belongs to the Wnt family. It is a proto-oncogene that functions in modulating cell fate and behavior during vertebrate development and has an important role in Wnt signaling pathway. It is a glycoprotein that plays an important role in cell communication and development. Wnt5A acts as a ligand for members of the frizzled family of seven transmembrane receptors. It is suspected that Wnt5A is a developmental protein. Wnt5A is a signaling molecule which affects the development of discrete regions of tissues and is likely to signal over only few cell diameters.

Long Name

Wingless-type MMTV Integration Site Family, Member 5a

Alternate Names

Wnt5a

Gene Symbol

WNT5A

UniProt

NP_003383 (Human)

Additional Wnt-5a Products

Product Documents for Wnt-5a Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Wnt-5a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Ligands (Wnts)

Citations for Wnt-5a Antibody - BSA Free

Customer Reviews for Wnt-5a Antibody - BSA Free (1)

4 out of 5
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Customer Images

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  • Wnt-5a Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Skin tissue and Skin frozen tissue
    Species: Petaurus breviceps (marsupial)
    Verified Customer | Posted 02/25/2019
    Epithelial expression of Wnt5a
    1:100 dilution. We cryo-sectioned fixed tissue (12um slices) in OCT, washed samples for 10min in PBT (PBS + 0.1% tween), blocked the sample for 1hr in 3%BSA/PBS and then incubated with the antibody overnight at 4C. The next day we washed 3x for 10min in PBT and incubated for one hr in Alexa488 (1:500), washed 3x10min in PBS and coverslipped.
    Wnt-5a Antibody - BSA Free NBP2-24752

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Protocols

View specific protocols for Wnt-5a Antibody - BSA Free (NBP2-24752):

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

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