Wnt-9a Antibody (HL2422) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25757

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2422 expressed in HEK293

Format

Azide and BSA Free
View all Wnt-9a Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide of human Wnt-9a.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Wnt-9a Antibody (HL2422) - Azide and BSA Free

Wnt-9a Antibody (HL2422) - Azide and BSA Free

Immunohistochemistry-Paraffin: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] -

Immunohistochemistry-Paraffin: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Wnt9a antibody [HL2422] detects secreted Wnt9a protein by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. Wnt9a stained by Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wnt-9a Antibody (HL2422) - Azide and BSA Free

Western Blot: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] -

Western Blot: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Wnt-9a Antibody (HL2422) - Azide and BSA Free

Western Blot: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] -

Western Blot: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Wnt-9a Antibody (HL2422) - Azide and BSA Free

Immunohistochemistry-Paraffin: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] -

Immunohistochemistry-Paraffin: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Wnt9a antibody [HL2422] detects secreted Wnt9a protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Wnt9a stained by Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wnt-9a Antibody (HL2422) - Azide and BSA Free

Western Blot: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] -

Western Blot: Wnt-9a Antibody (HL2422) - Azide and BSA Free [NBP3-25757] - Various whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (NBP3-25757) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Wnt-9a Antibody (HL2422) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Wnt-9a Antibody (HL2422) [NBP3-25757] -

Wnt-9a antibody [HL2422] detects Wnt-9a protein by immunofluorescent analysis.
Sample: T-47D cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wnt-9a stained by Wnt-9a antibody [HL2422] (NBP3-25757) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.

Applications for Wnt-9a Antibody (HL2422) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-9a

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 9A

Alternate Names

Wnt-14, Wnt9a

Gene Symbol

WNT9A

Additional Wnt-9a Products

Product Documents for Wnt-9a Antibody (HL2422) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Wnt-9a Antibody (HL2422) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Ligands (Wnts)

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Protocols

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