Recombinant Human FGF basic, 145 aa (TC Grade) Protein, CF

  (4 citations)
(1 Review)
    
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Citations (4)
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  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    This protein was used at 20-50 ng/mL to support a 100-fold expansion of rat cortical neural stem cells or mouse cortical stem cells. Cells were cultured in N2 plus medium and supplemented with fresh FGF basic every day. Medium was changed every other day and cells were split every 2-4 days upon becoming 70% confluent.
  • Source
    E. coli-derived human FGF basic protein
    Ala144-Ser288
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala144
  • Predicted Molecular Mass
    16 kDa
Product Datasheets

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4114-TC
 
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
 
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Background: FGF basic

FGF basic (also known as FGF-2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF-2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4-6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

  • References:
    1. Sorenson, V. et al. (2006) BioEssays 28:504.
    2. Kardami, E. et al. (2004) Cardiovasc. Res. 63:458.
    3. Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115.
    4. Abraham, J.A. et al. (1986) EMBO J. 5:2523.
    5. Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836.
    6. Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505.
    7. Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254.
    8. Arese, M. et al. (1999) Mol. Biol. Cell 10:1429.
    9. Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249.
    10. Nickel, W. (2005) Traffic 6:607.
    11. SwissProt # P09038.
    12. Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839.
    13. Bailly, K. et al. (2000) FASEB J. 14:333.
    14. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
  • Long Name:
    Fibroblast Growth Factor basic
  • Entrez Gene IDs:
    2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
  • Alternate Names:
    basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Sample Type
  1. Ursodeoxycholic acid inhibits the proliferation of colon cancer cells by regulating oxidative stress and cancer stem-like cell growth
    Authors: EK Kim, JH Cho, E Kim, YJ Kim
    PLoS ONE, 2017;12(7):e0181183.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. The FDA-approved drug sofosbuvir inhibits Zika virus infection
    Authors: Kristen M Bullard-Fe
    Antiviral Res, 2016;137(0):134-140.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells.
    Authors: Adepoju A, Micali N, Ogawa K, Hoeppner D, McKay R
    Stem Cells, 2014;32(3):770-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling.
    Authors: West FD, Machacek DW, Boyd NL, Pandiyan K, Robbins KR, Stice SL
    Stem Cells, 2008;26(11):2768-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

FAQs

  1. What receptors does FGF basic bind?

  2. Does human FGF basic show activity on mouse cells?
    • Yes, it does. The bioassay uses NR-6 mouse fibroblast cells.  There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

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Reconstitution Buffer 1 (PBS)

RB01
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