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Recombinant Human FGF basic, 145 aa (TC Grade) Protein, CF

4114-TC 4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4114-TC-01M
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Product Datasheets Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF basic, 145 aa (TC Grade) Protein, CF Summary

Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
This protein was used at 20-50 ng/mL to support a 100-fold expansion of rat cortical neural stem cells or mouse cortical stem cells. Cells were cultured in N2 plus medium and supplemented with fresh FGF basic every day. Medium was changed every other day and cells were split every 2-4 days upon becoming 70% confluent.
Source
E. coli-derived human FGF basic protein
Ala144-Ser288
Accession #
NP_001997
N-terminal Sequence
Analysis
Ala144
Predicted Molecular Mass
16 kDa

Product Datasheets

Product Datasheet
COA
SDS

4114-TC

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF basic

FGF basic (also known as FGF-2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF-2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4-6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

References
  1. Sorenson, V. et al. (2006) BioEssays 28:504.
  2. Kardami, E. et al. (2004) Cardiovasc. Res. 63:458.
  3. Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115.
  4. Abraham, J.A. et al. (1986) EMBO J. 5:2523.
  5. Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836.
  6. Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505.
  7. Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254.
  8. Arese, M. et al. (1999) Mol. Biol. Cell 10:1429.
  9. Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249.
  10. Nickel, W. (2005) Traffic 6:607.
  11. SwissProt # P09038.
  12. Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839.
  13. Bailly, K. et al. (2000) FASEB J. 14:333.
  14. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
Long Name
Fibroblast Growth Factor basic
Entrez Gene IDs
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin

Citations for Recombinant Human FGF basic, 145 aa (TC Grade) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Ursodeoxycholic acid inhibits the proliferation of colon cancer cells by regulating oxidative stress and cancer stem-like cell growth
    Authors: EK Kim, JH Cho, E Kim, YJ Kim
    PLoS ONE, 2017;12(7):e0181183.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The FDA-approved drug sofosbuvir inhibits Zika virus infection
    Authors: Kristen M Bullard-Fe
    Antiviral Res, 2016;137(0):134-140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells.
    Authors: Adepoju A, Micali N, Ogawa K, Hoeppner D, McKay R
    Stem Cells, 2014;32(3):770-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling.
    Authors: West FD, Machacek DW, Boyd NL, Pandiyan K, Robbins KR, Stice SL
    Stem Cells, 2008;26(11):2768-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What receptors does FGF basic bind?
  2. Does human FGF basic show activity on mouse cells?

    • Yes, it does. The bioassay uses NR-6 mouse fibroblast cells.  There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

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Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
1 Review

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Recombinant Human FGF basic, 145 aa (TC Grade) Protein, CF
By Anonymous on 05/26/2018
Application: Cell proliferation