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A Guide to the Colony Forming Cell Assay: Methods and Tips

Sunday, October 16, 2016 - 09:39

This video protocol provides step-by-step instructions on how to consistently perform the Colony Forming Cell (CFC) Assay. The colony forming cell assay, also commonly called the methylcellulose assay, is used in the study of hematopoietic stem cells to investigate their proliferative and differentiation potential. Using the tips provided throughout this video, researchers using the CFC assay will be able to more accurately evaluate and score colony formations.

This video includes:

  • An overview of the CFC Assay
  • How to aliquot methylcellulose
  • Cell preparation techniques
  • Tips for cell plating
  • Methods for cell counting
  • Tips for colony identification
  • Benefits of R&D...

5 Tips to Increase the Reproducibility of MSC and iPSC Differentiation

Tuesday, October 11, 2016 - 13:50

Mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs) are being utilized for the development of multitude of regenerative therapies. Establishing standards and practices for characterizing MSCs and iPSCs is an important quality issue for the future of stem cell research and regenerative medicine. Simple steps can be taken to improve the quality and efficiency of both MSC and iPSC expansion and differentiation.

Check out this article we wrote on Regmednet that provides 5 tips that could get you the quality and quantity of cells that you desire.

How to Non-invasively Monitor Stem Cell Differentiation using Luminex Technology

Monday, September 12, 2016 - 13:32

Joy Aho, PhD, Manager of Stem Cell Research and Development, Bio-Techne
Richard Fuerstenberg, Manager of Multiplex Development, Bio-Techne

In this webinar, Bio-Techne scientists, Richard Fuerstenberg and Joy Aho, discuss a novel method for monitoring stem cell pluripotency, expansion, and differentiation in vitro. This technology utilizes non-invasive sampling of cell culture supernatants to identify and follow changes in key secreted growth factors and cytokines. Using Proteome Profiler™ Antibody Arrays and Luminex® Assays, researchers can quickly monitor changes in cell phenotype without sacrificing yield. These techniques can be used to minimize cross-experiment variability during differentiation as well as to identify key analytes that could be used to predict or direct cell...

Organoids and Beyond – 3D Tissue in a Dish

Tuesday, August 30, 2016 - 13:40

Madeline Lancaster, PhD, MRC Laboratory of Molecular Biology
Omer Yilmaz, PhD, Assistant Professor of Biology, MIT
Sri Devi Narasimhan, Scientific Editor, Cell
Marie Bao, Editor, Developmental Cell

In this webinar, brought to you by Cell Press and Bio-Techne, we bring together leading researchers that are using stem cells to create organoids for the advancement of basic biology, regenerative medicine, and personalized medicine. With the growing use of organoids for these applications, this webinar discusses the challenges of using organoids to accurately recapitulate in vivo complexity, including microenvironment cues and cellular crosstalk. Topics in this webinar include:

Topics in this showcase...

How to Optimize and Standardize Stem Cell Differentiation

Friday, August 26, 2016 - 09:13

Joy Aho, PhD, Manager of Stem Cell Research and Development, Bio-Techne

In this webinar Dr. Joy Aho, Manager of Stem Cells Research and Development at Bio-Techne, discusses the importance of utilizing standardized differentiation protocols when using stem cells in regenerative medicine research or toxicology screening. Standardizing differentiation will help minimize cross-experiment variability and provide more robust and reliable data. Specific tools and techniques are showcased in this webinar that are geared to help both novice and expert researchers optimize and standardize their stem cell differentiation. High-throughput methods for verifying and analyzing stem cell differentiation is also discussed in this webinar.

Topics to be covered:

  • ...

Defining A Regulatory Path For Induced Pluripotent Stem Cells Based Cell Therapy

Friday, August 26, 2016 - 09:12

Kapil Bharti, PhD, National Eye Institute, Unit on Ocular Stem Cells and Translational Research

Induced pluripotent stem (iPS) cells are a potential source for developing autologous cell therapies. In this webinar, Dr. Kapil Bharti, provides a streamlined regulatory (cell manufacturing and preclinical) path towards filing an Investigational New Drug (IND) application to conduct a phase I clinical trial using autologous iPS cells. His group has developed a clinically compatible Good Manufacturing Practices (GMP) process to generate and functionally validate age-related macular degeneration (AMD) patient-specific iPS cell-derived retinal pigment monolayer tissue. He will discuss in-process quality controls that they have developed to monitor the entire manufacturing process and steps to validate patient-specific...

Learn More about Top Researchers with Our New Interview Series

Wednesday, August 24, 2016 - 03:51

Novus Biologicals is launching an interview series to learn more about the research being done by leaders in a variety of research areas. We also ask what drives these faculty members to do research and why they think their research is important to the general public. Look forward to a new interview each month!

Our first interview features Noboru Mizushima, Professor of Biochemistry and Molecular Biology at the University of Tokyo. Dr. Mizushima has made many critical findings in the field of autophagy and is one of the most highly cited researchers in his field. Learn more about Dr. Mizushima and find out what he had to say about his research and where he sees it moving in the future.

Tools to Optimize Pluripotent Stem Cell Cultures and Differentiation

Friday, August 12, 2016 - 13:37

Joy Aho, PhD, Manager of Stem Cell Research and Development, Bio-Techne
Scott Schachtele, PhD, Product Manager, Bio-Techne

This webinar is a recording of the Innovation Showcase presented by Bio-Techne at the 2016 Annual Meeting for the International Society for Stem Cell Research (ISSCR). This showcase starts with a brief discussion of strategies for optimizing your pluripotent stem cell workflow, including new data highlighting products that simplify cell characterization and differentiation. The second segment of the showcase focuses on exciting and progressive tools that can be used to improve stem cell culture and differentiation protocols. Topics in this showcase include:

  • Media supplements to optimize stem cell differentiation
  • ...

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