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This video protocol provides step-by-step instructions on how to consistently perform the Colony Forming Cell (CFC) Assay. The colony forming cell assay, also commonly called the methylcellulose assay, is used in the study of hematopoietic stem cells to investigate their proliferative and differentiation potential. Using the tips provided throughout this video, researchers using the CFC assay will be able to more accurately evaluate and score colony formations.

This video includes:

• An overview of the CFC Assay
• How to aliquot methylcellulose
• Cell preparation techniques
• Tips for cell plating
• Methods for cell counting
• Tips for colony identification
• Benefits of R&D...

Joy Aho, PhD, Manager of Stem Cell Research and Development, Bio-Techne
Richard Fuerstenberg, Manager of Multiplex Development, Bio-Techne

In this webinar, Bio-Techne scientists, Richard Fuerstenberg and Joy Aho, discuss a novel method for monitoring stem cell pluripotency, expansion, and differentiation in vitro. This technology utilizes non-invasive sampling of cell culture supernatants to identify and follow changes in key secreted growth factors and cytokines. Using Proteome Profiler™ Antibody Arrays and Luminex^® Assays, researchers can quickly monitor changes in cell phenotype without sacrificing yield. These techniques can be used to minimize cross-experiment variability during differentiation as well as to identify key analytes that could be used to predict or direct cell...

Joy Aho, PhD, Manager of Stem Cell Research and Development, Bio-Techne

In this webinar Dr. Joy Aho, Manager of Stem Cells Research and Development at Bio-Techne, discusses the importance of utilizing standardized differentiation protocols when using stem cells in regenerative medicine research or toxicology screening. Standardizing differentiation will help minimize cross-experiment variability and provide more robust and reliable data. Specific tools and techniques are showcased in this webinar that are geared to help both novice and expert researchers optimize and standardize their stem cell differentiation. High-throughput methods for verifying and analyzing stem cell differentiation is also discussed in this webinar.

Topics to be covered:

• ...

Novus Biologicals is launching an interview series to learn more about the research being done by leaders in a variety of research areas. We also ask what drives these faculty members to do research and why they think their research is important to the general public. Look forward to a new interview each month!

Our first interview features Noboru Mizushima, Professor of Biochemistry and Molecular Biology at the University of Tokyo. Dr. Mizushima has made many critical findings in the field of autophagy and is one of the most highly cited researchers in his field. Learn more about Dr. Mizushima and find out what he had to say about his research and where he sees it moving in the future.