ADAM8 Antibody

Novus Biologicals | Catalog # NB100-1017

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ADAM8 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QRKQGAGAPTAP corresponding to C-Terminus according to NP_001100.3, NP_001157962.1.

Localization

Cell Membrane

Specificity

This antibody is expected to recognise isoform 1 and 3 (NP_001100.3; NP_001157962.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Goat ADAM8 Antibody (NB100-1017) is a polyclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-ADAM8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ADAM8 Antibody

ADAM8 Antibody

Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] -

Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] - (1µg/ml) staining of Caco-2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
ADAM8 Antibody

Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] -

Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] - (1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ADAM8 Antibody

Flow Cytometry: Goat Polyclonal ADAM8 Antibody [NB100-1017] -

Flow Cytometry: Goat Polyclonal ADAM8 Antibody [NB100-1017] - Flow cytometric analysis of paraformaldehyde fixed Caco-2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
ADAM8 Antibody

Immunohistochemistry-Paraffin: Goat Polyclonal ADAM8 Antibody [NB100-1017] -

Immunohistochemistry-Paraffin: Goat Polyclonal ADAM8 Antibody [NB100-1017] - (6µg/ml) staining of paraffin embedded Human Lymph Node. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
ADAM8 Antibody

Control: Goat Polyclonal ADAM8 Antibody [NB100-1017] -

Control: Goat Polyclonal ADAM8 Antibody [NB100-1017] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

Applications for ADAM8 Antibody

Application
Recommended Usage

ELISA

Detection limit 1:16000

Flow Cytometry

10 ug/ml

Immunohistochemistry

6-8 ug/ml

Immunohistochemistry-Paraffin

6-8 ug/ml

Western Blot

1 - 3 ug/ml
Application Notes
Approx. 90kDa band observed in lysates of cell line Caco-2, approx. 80kDa in Human Duodenum lysates and approx. 85kDa in preliminary testing of MCF7 cell lysate (calculated MW of 88.8kDa according to NP_001100.3 and 78.8kDa according to NP_001157962.1).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ADAM8

Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. ADAM8 is involved in protein processing at the cell surface and is also thought to act as a leukocyte differentiation marker.

Long Name

A Disintegrin and Metalloprotease-like Domain 8

Alternate Names

CD156a, MS2

Gene Symbol

ADAM8

UniProt

P78325

Additional ADAM8 Products

Product Documents for ADAM8 Antibody

Certificate of Analysis

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Product Specific Notices for ADAM8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

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Protocols

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