CEACAM5/CD66e Antibody (5C8C4) - BSA Free

Novus Biologicals | Catalog # NBP2-54625

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5C8C4

Format

BSA Free
View all CEACAM-5/CD66e Primary Antibodies »

Product Specifications

Immunogen

Soluble human CEACAM5/CD66e

Specificity

This antibody recognizes human CEACAM 5. It does not cross-react with CEACAM-1, -3, -4, -6, -7, and -8 or rat CEACAM1, mouse CEACAM1 and mouse CEACAM2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CEACAM5/CD66e Antibody (5C8C4) - BSA Free (NBP2-54625) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CEACAM5/CD66e Antibody (5C8C4) - BSA Free

Western Blot: CEACAM5/CD66e Antibody (5C8C4)BSA Free [NBP2-54625]

Western Blot: CEACAM5/CD66e Antibody (5C8C4)BSA Free [NBP2-54625]

Western Blot: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM5 [5C8C4] followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.
Flow Cytometry: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]

Flow Cytometry: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]

Flow Cytometry: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM5 [5C8C4] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).
ELISA: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]

ELISA: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]

ELISA: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM5/8 [5C8C4] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Immunoprecipitation: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]

Immunoprecipitation: CEACAM5/CD66e Antibody (5C8C4) - BSA Free [NBP2-54625]

Immunoprecipitation: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM5/8 [5C8C4]. Detection of the sample using 5 ug/ml pAb Anti-CEA followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.

Applications for CEACAM5/CD66e Antibody (5C8C4) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CEACAM-5/CD66e

The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Alternate Names

CD66e, CEA, CEACAM5

Gene Symbol

CEACAM5

Additional CEACAM-5/CD66e Products

Product Documents for CEACAM5/CD66e Antibody (5C8C4) - BSA Free

Certificate of Analysis

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Product Specific Notices for CEACAM5/CD66e Antibody (5C8C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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