Cytochrome c Antibody (7H8.2C12) - BSA Free

Novus Biologicals | Catalog # NB100-56503

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila, Rabbit

Cited:

Human, Mouse, Rat, Insect - Drosophila, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Flow Cytometry Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 7H8.2C12

Format

BSA Free
View all Cytochrome c Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).

Marker

Mitochondria Marker

Specificity

This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Cytochrome c Antibody (7H8.2C12) - BSA Free

Western Blot: Cytochrome c Antibody (7H8.2C12)BSA Free [NB100-56503]

Western Blot: Cytochrome c Antibody (7H8.2C12)BSA Free [NB100-56503]

Cytochrome-c-Antibody-7H8-2C12-Western-Blot-NB100-56503-img0015.jpg
Immunocytochemistry/ Immunofluorescence: Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Immunocytochemistry/ Immunofluorescence: Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - p73 was detected in immersion fixed Hela human cell line using NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria.
Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog # NB100-56503) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).
Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Western Blot: Cytochrome c Antibody (7H8.2C12)BSA Free [NB100-56503]

Western Blot: Cytochrome c Antibody (7H8.2C12)BSA Free [NB100-56503]

Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Human HeLa lysate probed with Cytochrome C antibody at 0.1 ug/ml.
Western Blot: Cytochrome c Antibody (7H8.2C12)BSA Free [NB100-56503]

Western Blot: Cytochrome c Antibody (7H8.2C12)BSA Free [NB100-56503]

Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 ug of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Detection of Cytochrome C in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Cytochrome C Monoclonal Antibody (Catalog # NB100-56503, filled histogram), or Mouse IgG2B isotype control (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Flow Cytometry: Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503]

Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Simple Western: Cytochrome c Antibody (7H8.2C12)BSA Free [NB100-56503]

Simple Western: Cytochrome c Antibody (7H8.2C12)BSA Free [NB100-56503]

Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 ug/mL of Mouse Anti-Cytochrome c Monoclonal Antibody (Catalog # NB100-56503). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Cytochrome c Antibody (7H8.2C12) - BSA Free

Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] -

Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C Antibodies (NB100-56503SS), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 20 times. Image from verified customer review.
Cytochrome c Antibody (7H8.2C12) - BSA Free

Western Blot: Cytochrome c Antibody (7H8.2C12) - BSA Free [NB100-56503] -

Effects of resveratrol on the expression of SIRT1, PGC-1 alpha, HIF-1 alpha & apoptosis proteins in vivo. (A) Activation of the SIRT1–PGC-1 alpha –HIF-1 alpha signaling pathway in PC–AKI associated with DN in rabbits. Representative western blot images of proteins in the rabbits with DN treated with saline (Cont), resveratrol alone (Res), iohexol (PC–AKI), & co-treatment with resveratrol & iohexol (Res+PC–AKI). (B–H) Relative densitometry analysis of the ratios of SIRT1–PGC-1 alpha –HIF-1 alpha signaling proteins to beta -actin were expressed as mean ± standard error. *P < 0.05 vs. Cont; #P < 0.05 vs. PC–AKI. Cont, control; Res, resveratrol; PC–AKI, post-contrast acute kidney injury; SIRT1, silent information regulator l; HIF-1 alpha, hypoxia-inducible transcription factor-1 alpha ; DN, diabetic nephropathy; PGC-1 alpha, peroxisome proliferator-activated receptor gamma coactivator-1 alpha. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31402864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Cytochrome c Antibody (7H8.2C12) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 16467206)

Flow (Intracellular)

reported in scientific literature (Mohr et al (2004))

Flow Cytometry

1:20-1:2000

Immunoblotting

reported in scientific literature (PMID 16436379)

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (Yamasaki et al (2006))

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500. Use reported in scientific literature (Fujimara et al (1998))

Simple Western

2.5 ug/ml

Western Blot

0.05-0.5 ug/ml
Application Notes
An approx. 15 kDa band is observed. See Simple Western Antibody Database for Simple Western validation: tested in human heart tissue lysate; separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 2.5 ug/ml; matrix was 12-230 kDa.

Reviewed Applications

Read 1 review rated 5 using NB100-56503 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome c

In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3.

Alternate Names

CYCS

Entrez Gene IDs

54205 (Human); 13063 (Mouse); 25309 (Rat)

Gene Symbol

CYCS

UniProt

P99999 (Human)

Additional Cytochrome c Products

Product Documents for Cytochrome c Antibody (7H8.2C12) - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytochrome c Antibody (7H8.2C12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Cytochrome c Antibody (7H8.2C12) - BSA Free

Customer Reviews for Cytochrome c Antibody (7H8.2C12) - BSA Free (1)

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  • Cytochrome c Antibody (7H8.2C12) - BSA Free
    Name: Anonymous
    Application: Simple Western
    Sample Tested: human brain lysate
    Species: Human
    Verified Customer | Posted 04/06/2023
    Cytochrome C Antibodies NB100-56503SS, ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 20 times.
    Cytochrome c Antibody (7H8.2C12) - BSA Free NB100-56503

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