GLI-1 Antibody (1B9F8) - BSA Free
Novus Biologicals | Catalog # NBP2-52475
![Western Blot: GLI-1 Antibody (1B9F8)BSA Free [NBP2-52475]](https://beta-resources.rndsystems.com/images/products/GLI-1-Antibody-1B9F8-Western-Blot-NBP2-52475-img0005.jpg "Western Blot: GLI-1 Antibody (1B9F8)BSA Free [NBP2-52475]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1B9F8
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human GLI-1 (AA: 284-449) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse GLI-1 Antibody (1B9F8) - BSA Free (NBP2-52475) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for GLI-1 Antibody (1B9F8) - BSA Free
Western Blot: GLI-1 Antibody (1B9F8)BSA Free [NBP2-52475]
Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)![Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]](https://beta-resources.rndsystems.com/images/products/GLI-1-Antibody-1B9F8-Immunofluorescence-NBP2-52475-img0002.jpg "Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]")
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]
Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of Hela cells using GLI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.![Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]](https://beta-resources.rndsystems.com/images/products/GLI-1-Antibody-1B9F8-Immunohistochemistry-NBP2-52475-img0004.jpg "Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]")
Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]
Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of esophageal cancer tissues using GLI1 mouse mAb with DAB staining.![Flow Cytometry: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]](https://beta-resources.rndsystems.com/images/products/GLI-1-Antibody-1B9F8-Flow-Cytometry-NBP2-52475-img0001.jpg "Flow Cytometry: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]")
Flow Cytometry: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]
Flow Cytometry: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of HepG2 cells using GLI1 mouse mAb (green) and negative control (red).![Western Blot: GLI-1 Antibody (1B9F8)BSA Free [NBP2-52475]](https://beta-resources.rndsystems.com/images/products/GLI-1-Antibody-1B9F8-Western-Blot-NBP2-52475-img0006.jpg "Western Blot: GLI-1 Antibody (1B9F8)BSA Free [NBP2-52475]")
Western Blot: GLI-1 Antibody (1B9F8)BSA Free [NBP2-52475]
Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against HEK293 (1) and GLI1 (AA: 284-449)-hIgGFc transfected HEK293 (2) cell lysate.![Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]](https://beta-resources.rndsystems.com/images/products/GLI-1-Antibody-1B9F8-Immunohistochemistry-NBP2-52475-img0003.jpg "Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]")
Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]
Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of ovarian cancer tissues using GLI1 mouse mAb with DAB staining.![ELISA: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]](https://beta-resources.rndsystems.com/images/products/GLI-1-Antibody-1B9F8-ELISA-NBP2-52475-img0008.jpg "ELISA: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]")
ELISA: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]
ELISA: GLI-1 Antibody (1B9F8) [NBP2-52475] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).Applications for GLI-1 Antibody (1B9F8) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This antibody is Cytof ready.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GLI-1
Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Alternate Names
GLI1
Gene Symbol
GLI1
Additional GLI-1 Products
Product Documents for GLI-1 Antibody (1B9F8) - BSA Free
Product Specific Notices for GLI-1 Antibody (1B9F8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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