HSP27 Antibody (5D7)

Novus Biologicals | Catalog # NBP2-37599

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D7
View all HSP27 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human HSP27 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse HSP27 Antibody (5D7) (NBP2-37599) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HSP27 Antibody (5D7)

Western Blot: HSP27 Antibody (5D7)BSA Free [NBP2-37599]

Western Blot: HSP27 Antibody (5D7)BSA Free [NBP2-37599]

Western Blot: HSP27 Antibody (5D7) [NBP2-37599] - Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

Immunocytochemistry/ Immunofluorescence: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

Immunocytochemistry/Immunofluorescence: HSP27 Antibody (5D7) [NBP2-37599] - Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

Immunohistochemistry: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

Immunohistochemistry: HSP27 Antibody (5D7) [NBP2-37599] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB staining.
Flow Cytometry: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

Flow Cytometry: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

Flow Cytometry: HSP27 Antibody (5D7) [NBP2-37599] - Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple).
Immunohistochemistry: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

Immunohistochemistry: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

Immunohistochemistry: HSP27 Antibody (5D7) [NBP2-37599] - Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining.
ELISA: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

ELISA: HSP27 Antibody (5D7) - BSA Free [NBP2-37599]

ELISA: HSP27 Antibody (5D7) [NBP2-37599] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for HSP27 Antibody (5D7)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for HSP27 Antibody (5D7)

Certificate of Analysis

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Product Specific Notices for HSP27 Antibody (5D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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