Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 561819
View all ADAMTS15 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS15
Gly18-Cys682
Accession # Q8TE58

Specificity

Detects human ADAMTS15 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ADAMTS15 Antibody

Detection of Human ADAMTS15 antibody by Western Blot.

Detection of Human ADAMTS15 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for mature ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ADAMTS15 antibody in Human Kidney by Immunohistochemistry (IHC-P).

ADAMTS15 in Human Kidney.

ADAMTS15 was detected in immersion fixed paraffin-embedded sections of human kidney using Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of ADAMTS15 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of ADAMTS15 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Applications for Human ADAMTS15 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: MCF‑7 human breast cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

2 µg/mL
Sample: MCF‑7 human breast cancer cell line

Reviewed Applications

Read 3 reviews rated 4.3 using MAB5149 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAMTS15

A disintegrin and metalloproteinase with thrombospondin motifs 15 (ADAMTS15) is a secreted multi-domain protease that is primarily expressed in fetal liver and kidney. ADAMTS1, 4, 5, 8, and 15 form a subfamily of ADAMTS proteases that possess aggrecanase activity. These proteins are synthesized as zymogens which have a pro-domain that is removed by furin-like protein convertases. ADAMTS15 functions as a suppressor of tumor growth and invasion. It is downregulated in colon cancer, and its expression in breast cancer correlates with poor prognosis. Within amino acids (aa) 18-682 (which includes the propeptide, peptidase, disintegrin, first TSP-1 and most of the Cys-rich domain), human ADAMTS15 shares 95% aa sequence identity with mouse and rat ADAMTS15.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 15

Alternate Names

A disintegrin and metalloproteinase with thrombospondin motifs 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, preproprotein, ADAM metallopeptidase with thrombospondin type 1 motif, 15, ADAM-TS 15, ADAM-TS15, ADAMTS-15, EC 3.4.24, EC 3.4.24.-, EC 3.4.24.14, EC 3.4.24.82, metalloprotease disintegrin 15 with thrombospondin domains, MGC126403

Entrez Gene IDs

170689 (Human); 235130 (Mouse); 300474 (Rat)

Gene Symbol

ADAMTS15

UniProt

Q8TE58

Additional ADAMTS15 Products

Product Documents for Human ADAMTS15 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAMTS15 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human ADAMTS15 Antibody

Customer Reviews for Human ADAMTS15 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 3 的 3 reviews Showing All
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  • Human ADAMTS15 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Human
    Verified Customer | Posted 07/19/2022
    Human ADAMTS15 Antibody MAB5149
  • Human ADAMTS15 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 09/23/2019
    I used this antibody for developing a sandwich ELISA in combination with pAb (cat. AF5149) and protein (cat. 5149). This combination works well for a sandwich ELISA detection human ADAMTS 15 in serum.
    Human ADAMTS15 Antibody MAB5149
  • Human ADAMTS15 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 09/23/2019
    I used this protein as a standard for developing an ELISA in combination with pAb (cat. AF5149) and mAb (cat. MAB 5149). This combination works well for a sandwich ELISA detection human ADAMTS 14 in serum.
    Human ADAMTS15 Antibody MAB5149

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Protocols

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