Human ALCAM/CD166 Antibody [Biotin]

Name: Human ALCAM/CD166 Antibody [Biotin]
Brand: R&D Systems
SKU: BAF656
Price: 529 USD
Availability: InStock

R&D Systems | Catalog # BAF656

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all ALCAM/CD166 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # AAB59499

Specificity

Detects human ALCAM/CD166 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse ALCAM, recombinant human (rh) ICAM-2, rhICAM-3, rhE-Selectin, and rhP-Selectin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ALCAM/CD166 Antibody [Biotin]

Human ALCAM / CD166 ELISA Standard Curve

Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 656-AL) was serially diluted and captured by Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human ALCAM/CD166 Antibody [Biotin]

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 656-AL)

Human ALCAM/CD166 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human ALCAM/CD166 Antibody (Catalog # MAB6561)
Standard: Recombinant Human ALCAM Fc Chimera Protein, CF (Catalog # 656-AL)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be up‑regulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.