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Human/Canine Integrin  beta 1/CD29 Antibody

Catalog #: AF1778
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AF1778
AF1778-SP

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Detection of Human Integrin beta 1/CD29 by Western Blot.
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Human/Canine Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Human, Canine
Specificity
Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1 isoform 1A
Gln21-Asp728
Accession # P05556
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
MG‑63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Simple Western
10-20 µg/mL
HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U‑87 MG human glioblastoma/astrocytoma cell line
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Knockout Validated
Integrin  beta 1/CD29 is specifically detected in A549 human lung carcinoma parental cell line but is not detectable in Integrin  beta 1/CD29 knockout A549 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Integrin beta 1/CD29 antibody by Western Blot. View Larger

Detection of Human Integrin beta 1/CD29 by Western Blot. Western blot shows lysates of MG-63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta 1/CD29 at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human Integrin  beta 1/CD29 by Simple WesternTM. Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin  beta 1/CD29 at approximately 198 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Canine Integrin  beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in Canine PBMCs antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Canine PBMCs by Flow Cytometry. Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in Canine Mesenchymal Stem Cells antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Canine Mesenchymal Stem Cells by Flow Cytometry. Canine mesenchymal stem cells were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry Integrin beta 1/CD29 antibody in Canine Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Integrin beta 1/CD29 in Canine Mesenchymal Stem Cells. Integrin beta 1/CD29 was detected in immersion fixed canine mesenchymal stem cells using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry Integrin beta 1/CD29 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Integrin beta 1/CD29 in Human Mesenchymal Stem Cells. Integrin beta 1/CD29 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Simple Western Detection of Human Integrin beta 1/CD29 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Integrin beta 1/CD29 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Integrin beta 1/CD29 at approximately 157-176 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry View Larger

Integrin  beta 1/CD29 in A549 Human Cell Line. Integrin  beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Canine Integrin  beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Knockout Validated Western Blot Shows Human Integrin beta 1/CD29 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Integrin beta 1/CD29 Specificity by Using Knockout Cell Line. Western blot shows lysates of A549 human lung carcinoma parental cell line and Integrin beta 1/CD29 knockout A549 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta 1/CD29 at approximately 105 kDa and 120 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

Integrin beta 1, also called CD29 and VLA-beta chain, associates with at least ten different integrin alpha subunits to form various VLA complexes. The beta 1 subunit has a broad tissue distribution except erythrocytes. Over aa 21-720, human  Integrin beta 1 shares 95% aa identity with canine Integrin beta 1.

Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

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Citations for Human/Canine Integrin  beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. PAI-1 uncouples integrin-?1 from restrain by membrane-bound ?-catenin to promote collagen fibril remodeling in obesity-related neoplasms
    Authors: Lin, LL;Nayak, B;Osmulski, PA;Wang, E;Wang, CP;Valente, PT;Wang, CM;Tan, X;Santanam, N;Wang, TL;Gaczynska, ME;Kost, ER;Huang, TH;Kirma, NB;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry, Functional Assay
  2. The extracellular niche and tumor microenvironment enhance KRAS inhibitor efficacy in pancreatic cancer
    Authors: Kumarasamy, V;Wang, J;Frangou, C;Wan, Y;Dynka, A;Rosenheck, H;Dey, P;Abel, EV;Knudsen, ES;Witkiewicz, AK;
    Cancer research
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Reparative effect of mesenchymal stromal cells on endothelial cells after hypoxic and inflammatory injury
    Authors: JM Sierra-Par, A Merino, M Eijken, H Leuvenink, R Ploeg, BK Møller, B Jespersen, CC Baan, MJ Hoogduijn
    Stem Cell Res Ther, 2020-08-12;11(1):352.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Desmocollin-2 promotes intestinal mucosal repair by controlling integrin-dependent cell adhesion and migration
    Authors: Sven Flemming, Anny-Claude Luissint, Dennis H. M. Kusters, Arturo Raya-Sandino, Shuling Fan, Dennis W. Zhou et al.
    Molecular Biology of the Cell
  5. Bystander cells enhance NK cytotoxic efficiency by reducing search time
    Authors: X Zhou, R Zhao, K Schwarz, M Mangeat, EC Schwarz, M Hamed, I Bogeski, V Helms, H Rieger, B Qu
    Sci Rep, 2017-03-13;7(0):44357.
    Species: Human
    Sample Types: Whole Cells
    Applications: Migration Assay
  6. In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
    Authors: Windus L, Kiss D, Glover T, Avery V
    Exp Cell Res, 2012-07-27;318(19):2507-19.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells
    Authors: Rickard J Storm
    J. Virol, 2017-02-14;0(0):.

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