Home / Integrin beta 1/CD29 / Human Integrin  beta 1/CD29 Biotinylated Antibody

Human Integrin  beta 1/CD29 Biotinylated Antibody

Catalog #: BAF1778
6 Citations Datasheet / COA / SDS
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BAF1778

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Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.
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Human Integrin  beta 1/CD29 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Integrin  beta 1 is observed and less than 1% cross-reactivity recombinant human (rh) Integrin  beta 2, rhIntegrin  beta 3, rmIntegrin  beta 6, and rmIntegrin  beta 7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1 isoform 1A
Gln21-Asp728
Accession # P05556
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Integrin  beta 1/CD29
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human Integrin beta 1/CD29 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1778, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

Integrin beta 1, also called CD29 and VLA-beta chain, associates with at least ten different integrin alpha subunits to form various VLA complexes. The beta 1 subunit has a broad tissue distribution except erythrocytes.

Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

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Citations for Human Integrin  beta 1/CD29 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. An optimized isolation of biotinylated cell surface proteins reveals novel players in cancer metastasis.
    Authors: Karhemo PR, Ravela S, Laakso M et al.
    J Proteomics.
  2. A recombinant technique for mapping functional sites of hetero-trimeric collagen helices: collagen IV CB3 fragment as a prototype for integrin binding
    Authors: Boudko, SP;Konopka, EH;Kim, W;Taga, Y;Mizuno, K;Springer, TA;Hudson, BG;Moy, TI;Lin, FY;
    The Journal of biological chemistry
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  3. Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin alpha5beta1 Is a Modulator of Extravillous Trophoblast Functions
    Authors: S Rattila, CEE Dunk, M Im, O Grichenko, Y Zhou, M Yanez-Mo, SM Blois, KM Yamada, O Erez, N Gomez-Lope, SJ Lye, B Hinz, R Romero, M Cohen, G Dveksler
    Cells, 2019-10-31;8(11):.
    Species: Human
    Sample Types:
    Applications: ELISA Detection
  4. Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
    Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
    J. Clin. Invest., 2019-05-14;129(7):2745-2759.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  5. Monoclonal antibodies to human laminin alpha4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of alpha6beta1 integrin and MCAM to alpha4-laminins.
    Authors: Ishikawa T, Wondimu Z, Oikawa Y, Ingerpuu S, Virtanen I, Patarroyo M
    Matrix Biol, 2014-03-27;36(0):5-14.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development (Detection)
  6. CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
    Authors: Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E
    Leukemia, 2011-12-02;26(5):1098-105.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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