Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody

R&D Systems | Catalog # FAB9577B

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry

Cited:

Immunohistochemistry, Flow Cytometry, Cell Selection, Flow Cytomery, Functional Assay, Purification

Label

Biotin

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu1
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Human EGF R/ErbB1

Specificity

Detects human EGFR based on Cetuximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Cetuximab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images

Detection of EGF R/ErbB1 antibody in A431 human epithelial carcinoma cell line antibody by Flow Cytometry.

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B, filled histogram) or irrelevant biotinylated antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.
EGFR antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

EGFR in A431 Human Cell Line.

EGFR was detected in unfixed live A431 human epithelial carcinoma cell line using Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: A431 human epithelial carcinoma cell line

Immunocytochemistry

8-25 µg/mL

Reviewed Applications

Read 1 review rated 4 using FAB9577B in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EGFR

Cetuximab is a biosimilar directed against the extracellular domain of EGFR. The biotinylated antibody can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices

For research use only

Citations for Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody

Customer Reviews (1)

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  • Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
    Name: Robert Berahovich
    Application: Flow Cytometry
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 09/29/2020
    Red histogram: HEK293 cells Blue histogram: HEK293 cells transduced to express a protein containing the EGFR tag
    Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody FAB9577B

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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  • Q: What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?

    A: Catalog #s MAB9577 and FAB9577 have a kappa light chain.

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