Human ErbB2/Her2 Biotinylated Antibody Summary
Thr23-Thr652
Accession # P04626
Applications
Human ErbB2/Her2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
ErbB2/Her2 in MCF-7 Human Cell Line. ErbB2/Her2 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human ErbB2/Her2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1129) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
View Larger
ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human ErbB2/Her2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ErbB2/Her2
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).
- Coussens, L. et al. (1985) Science 230:1132.
- Yamamoto, T. et al. (1986) Nature 319:230.
- Kanai, Y. et al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
- Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
- Carraway, K.L. 3rd et al. (1994) J. Biol. Chem. 269:14303.
- Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
- Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
- Schlessinger, J. (2000) Cell 103:211.
- Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
- Daly, R.J. (1999) Growth Factors 16:255.
Product Datasheets
Citations for Human ErbB2/Her2 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
Filter your results:
Filter by:
-
Covalent Hemin/G4 complex-linked sandwich bioassay on magnetic beads for femtomolar HER-2/neu detection in human serum via direct electrocatalytic reduction of oxygen
Authors: A Díaz-Ferná, EE Ferapontov
Oncogene, 2022-06-06;1219(0):340049.
Species: N/A
Sample Types: Beads
Applications: Functional Assay -
Immuno-Acoustic Sorting of Disease-Specific Extracellular Vesicles by Acoustophoretic Force
Authors: J Liu, Y Qu, H Wang
Micromachines, 2021-12-09;12(12):.
Species: Human
Sample Types: Protein
Applications: ELISA Capture -
Femtomolar electroanalysis of a breast cancer biomarker HER-2/neu protein in human serum by the cellulase-linked sandwich assay on magnetic beads
Authors: K Malecka, D Pankratov, EE Ferapontov
Anal. Chim. Acta, 2019-05-25;1077(0):140-149.
Species: Human
Sample Types: Serum
Applications: ELISA Capture, ELISA Detection -
Molecular nanoshearing: an innovative approach to shear off molecules with AC-induced nanoscopic fluid flow.
Authors: Shiddiky, Muhammad, Vaidyanathan, Ramanath, Rauf, Sakandar, Tay, Zhikai, Trau, Matt
Sci Rep, 2014-01-16;4(0):3716.
Species: Human
Sample Types: Serum
Applications: Functional Assay -
Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
Authors: Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA
Assay Drug Dev Technol, 2010-02-01;8(1):27-36.
Species: Human
Sample Types: Cell Lysates
Applications: Luminex Development, Western Blot -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Staining Reagents
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Human ErbB2/Her2 Biotinylated Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human ErbB2/Her2 Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
SK-BR-3 cell staining with Biotin-labeled pAb (BAF1129) and Streptavidin, Alexa Fluor® 633 conjugate.
