Human Fibronectin Antibody (P1H11)

R&D Systems | Catalog # MAB1918

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Canine

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P1H11
View all Fibronectin Primary Antibodies »

Product Specifications

Immunogen

Human plasma-derived Fibronectin

Specificity

Detects human Fibronectin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fibronectin Antibody (P1H11)

Detection of Fibronectin in HepG2 cells by Flow Cytometry

HepG2 (filled histogram) and Jurkat (open histogram) cells were stained with Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918, filled histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human Fibronectin by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Fibronectin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human Fibronectin by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (PC‑3) (NBP2-49856), Exosome Standards (Human Urine) (NBP2-49840) and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Fibronectin at approximately 290 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.
Fibronectin antibody in HepG2 and Daudi Human Cell Lines by Immunocytochemistry (ICC).

Fibronectin in HepG2 and Daudi Human Cell Lines.

Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining; left panel) and Daudi human Burkitt's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Fibronectin antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Fibronectin in Human Breast Cancer Tissue.

Fibronectin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and fibroblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Fibronectin by Western Blot

Detection of Fibronectin by Western Blot

DCN inhibits E-cadherin expression and EGFR pathway activation in IBC.a DCN suppresses E-cadherin expression and EGFR signaling in IBC cells. Expression levels of E-cadherin and EGFR are decreased in DCN-overexpressing MDA-IBC3, SUM190, SUM149, and BCX010 cells; also, the phosphorylation of EGFR (pEGFR) and ERK1/2 (pERK1/2) was suppressed in DCN-overexpressing IBC cell lines. Total ERK1/2 (tERK1/2) remains unchanged. GAPDH served as a loading control. b Treatment of IBC cells with DCN protein (4 or 8 μg/mL) for 2 h suppresses E-cadherin expression and EGFR pathway activation. Tubulin served as a loading control. c and d Western blot validation of E-cadherin and EGFR downregulation in tumor samples obtained from mammary fatpad transplantation of control or DCN-overexpressing MDA-IBC3 (c) or SUM149 (d) cells. e and f Immunohistochemical staining validation of E-cadherin and EGFR downregulation in tumor samples obtained from mammary fatpad transplantation of control or DCN-overexpressing MDA-IBC3 (e) or SUM149 (f) cells. Scale bar: 100 μm. g DCN inhibits EGFR signaling in IBC cells independently of EGF stimulation. DCN-overexpressing and control IBC cell lines were stimulated with 50 ng/mL EGF for the indicated number of hours, and total cell lysates were analyzed by western blotting. Both the total levels and the phosphorylation levels of EGFR and ERK1/2 were detected by western blotting. Tubulin served as a loading control. h DCN-mediated inhibition of E-cadherin does not affect expression of epithelial–mesenchymal transition markers. Cell lysates containing 40 μg of total protein were analyzed by western blotting with anti-E-cadherin, fibronectin, vimentin, and DCN antibodies. GAPDH served as a loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33452400), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Fibronectin Antibody (P1H11)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human intestine, kidney, and liver, and human breast cancer tissue

Immunoprecipitation

Yu, H. et al. (2004) Mol. Carcinog. 41:85.

Simple Western

10 µg/mL
Sample: Exosome Standards (PC-3) (Catalog # NBP2-49856), Exosome Standards (Human Urine) (Catalog # NBP2-49840) and HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line

Reviewed Applications

Read 7 reviews rated 4.4 using MAB1918 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fibronectin

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Human Fibronectin Antibody (P1H11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fibronectin Antibody (P1H11)

For research use only

Citations for Human Fibronectin Antibody (P1H11)

Customer Reviews for Human Fibronectin Antibody (P1H11) (7)

4.4 out of 5
7 Customer Ratings
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Showing  1 - 5 的 7 reviews Showing All
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  • Human Fibronectin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Cardiac fibroblasts
    Species: Human
    Verified Customer | Posted 07/08/2022
    Human Fibronectin Antibody (P1H11) MAB1918
  • Human Fibronectin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 09/28/2021
    Human Fibronectin Antibody (P1H11) MAB1918
  • Human Fibronectin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 05/15/2020
    tested the antibody in whole cell ELISA using fibronectin overexpression cells. It works well.
    Human Fibronectin Antibody (P1H11) MAB1918
  • Human Fibronectin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 10/01/2019
    Paired with AF1918
    Human Fibronectin Antibody (P1H11) MAB1918
  • Human Fibronectin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 08/12/2019
    Human Fibronectin Antibody (P1H11) MAB1918
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22321604
    Species: Human
    Verified Customer | Posted 02/12/2015
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 22225866
    Species: Human
    Verified Customer | Posted 02/12/2015

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