Human IL-6 Antibody (1936R)

R&D Systems | Catalog # MAB2061R

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Neutralization, Intracellular Staining by Flow Cytometry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 1936R
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-6
Val30-Met212
Accession # P05231

Specificity

Detects human IL-6 in direct ELISAs. Does not cross-react with recombinant IL-6 from mouse, rat, or pig.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-6 Antibody (1936R)

Detection of IL-6 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of IL‑6 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with 100 ng/mL LPS for 24 hours were stained with Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061R, filled histogram) or isotype control antibody (MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
Cell Proliferation Induced by IL-6 and Neutralization by Human IL-6 Antibody.

Cell Proliferation Induced by IL-6 and Neutralization by Human IL-6 Antibody.

Recombinant Human IL-6 (206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061R). The ND50 is typically 8.00-80.0 ng/mL.

Detection of IL‑6 in PBMC cells by Flow Cytometry.

PBMC stimulated with 100 ng/ml of LPS and Brefeldin A for 24 hrs (A) or unstimulated (B) were stained with Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061R) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.
Detection of IL-6 by Western Blot

Detection of IL-6 by Western Blot

The Vpr-TET2 axis independently enhances HIV-1 replication through reduced IFITM3 and sustained IL-6 expression. (A) Vpr-enhanced Env processing is independent of IL-6 signaling. MDMs were infected with HIV-1 or HIV-1 delta Vpr viruses for 4 days, with NVP added at 2 dpi, and treated with the isotype control or anti-IL-6 neutralizing antibody. Western blots were performed with cell lysates. Env proteins gp160 and gp120 were detected with anti-gp120 antibody, gp41 was detected by anti-gp41 antibody, and p24 was detected by anti-p24 antibody. gp120/p24 and gp120/gp160 ratios are shown. (B) MDMs transduced with control shRNA, shTET2, and shIFITM3 were infected with HIV-1 or HIV-1 delta Vpr and treated with the isotype control or anti-IL-6 neutralizing antibody. p24 levels in the supernatant were measured at 6 dpi. (C) Relative ability of Vpr to enhance HIV-1 replication in cells treated with the isotype control and anti-IL-6 neutralizing antibody at day 6. Data from two different donors are summarized by setting Vpr-enhanced replication in isotype control MDMs transduced with control shRNA at 1.0. * indicates P < 0.05. (D) The Vpr-TET2 axis enhances HIV-1 replication in macrophages independently by modulating expression of IFITM3 and IL-6 during HIV-1 infection. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31431548), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL-6 Antibody (1936R)

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: PBMCs treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and PBMC stimulated with 100 ng/ml of LPS and Brefeldin A for 24 hrs or unstimulated

Neutralization

Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. The Neutralization Dose (ND50) is typically 8.00-80.0 ng/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Reviewed Applications

Read 2 reviews rated 5 using MAB2061R in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

UniProt

P05231

Additional IL-6 Products

Product Documents for Human IL-6 Antibody (1936R)

Certificate of Analysis

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Product Specific Notices for Human IL-6 Antibody (1936R)

For research use only

Citations for Human IL-6 Antibody (1936R)

Customer Reviews for Human IL-6 Antibody (1936R) (2)

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Showing  1 - 2 的 2 reviews Showing All
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  • Human IL-6 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 12/13/2021
    Human IL-6 Antibody (1936R) MAB2061R
  • Human IL-6 Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: macrophage
    Species: Human
    Verified Customer | Posted 07/30/2021
    Human IL-6 Antibody (1936R) MAB2061R

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