Human LRIG1 Antibody (789211)

R&D Systems | Catalog # MAB7498

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 789211
View all LRIG1 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human LRIG1
Ala35-Ser779
Accession # Q96JA1

Specificity

Detects human LRIG1 in ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse LRIG1 and recombinant human LRIG3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human LRIG1 Antibody (789211)

Detection of LRIG1 antibody in LNCaP Human Cell Line antibody by Flow Cytometry.

Detection of LRIG1 in LNCaP Human Cell Line by Flow Cytometry.

LNCaP human prostate cancer cell line was stained with Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
LRIG1 antibody in SK-BR-3 Human Cell Line by Immunocytochemistry (ICC).

LRIG1 in SK‑BR‑3 Human Cell Line.

LRIG1 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
LRIG1 antibody in Human Kidney by Immunohistochemistry (IHC-P).

LRIG1 in Human Kidney.

LRIG1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of LRIG1 by Immunohistochemistry

Detection of LRIG1 by Immunohistochemistry

Increased stem cell and proliferative markers after daily applications of APE formulation in ex vivo cultures. After 5 days of daily topical application on skin explants of a formulation containing 1% APE or placebo, the organ cultures were fixed and frozen or paraffin-embedded. (A) Expression of beta 1-integrin (ITGB1), Collagen XVII (Coll XVII), Keratin 15 (KRT15), and LRIG1 was evaluated by IF. Arrow highlights the observed increase. Quantification of the expression level was performed using the Fiji software. Counterstaining of the nuclei was performed using Hoechst. (B) Expression of proliferative markers, Ki-67 and Survivin, was evaluated by IHC staining. Quantification of expression levels was performed using the Fiji software. Counterstaining of the nuclei in the Survivin samples was performed using hematoxylin. Statistical significance is indicated as follows: p < 0.05 (*), p < 0.001 (***), and p < 0.0001 (****). Scale bar = 100 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2073-4409/14/15/1176), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human LRIG1 Antibody (789211)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: LNCaP Human Cell Line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SK‑BR‑3 human breast cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Reviewed Applications

Read 3 reviews rated 3.7 using MAB7498 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LRIG1

LRIG1 (leucine-rich repeats and Ig-like domains-1; also LIG-1) is an approximately 134-145 kDa glycoprotein that belongs to the LRIG gene family. It is widely expressed and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and neurons. LRIG1 is believed to negatively regulate the ErbB family of receptors. In particular, and in a ligand-independent manner, LRIG1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number. Alternatively, LRIG1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction. Mature human LRIG1 is a 1059 amino acid (aa) type I transmembrane protein. It contains a large 760 amino acid (aa) extracellular domain (ECD) (aa 35-794) plus a 278 aa cytoplasmic region. The ECD contains 17 LRRs (aa 35‑491) and three C2-type Ig-like domains (aa 495-780). These two domain types are each sufficient for EGFR binding. There are two potential alternative splice forms. One contains a 27 aa insertion after Gly874, while another shows a 24 aa insertion after Lys387 coupled to a Gln substitution for aa 644-691. The LRIG1 ECD undergoes proteolysis, generating 100-110 and 55-60 kDa soluble fragments. Over aa 35‑779, human LRIG1 shares 90% aa sequence identity with mouse LRIG1.

Long Name

Leucine-rich Repeats and Immunoglobulin-like Domains 1

Alternate Names

Img, LIG1

Entrez Gene IDs

26018 (Human); 16206 (Mouse); 312574 (Rat)

Gene Symbol

LRIG1

UniProt

Q96JA1

Additional LRIG1 Products

Product Documents for Human LRIG1 Antibody (789211)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human LRIG1 Antibody (789211)

For research use only

Related Research Areas

EGF Family

Citations for Human LRIG1 Antibody (789211)

Customer Reviews for Human LRIG1 Antibody (789211) (3)

3.7 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
0%
3 Stars
67%
2 Stars
0%
1 Stars
0%

Have you used Human LRIG1 Antibody (789211)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 的 3 reviews Showing All
Filter By:
Clear Filters X
  • Human LRIG1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 09/18/2023
    A very fain band appeared at 150 kDa.
    Human LRIG1 Antibody (789211) MAB7498
  • Human LRIG1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Gastric epithelium
    Species: Human
    Verified Customer | Posted 10/30/2021
    Human LRIG1 Antibody (789211) MAB7498
  • Human LRIG1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood lymphocytes (PBL)
    Species: Human
    Verified Customer | Posted 06/28/2016
    Used with anti-mouse secondary in PE channel for detection. Could not see shift above background staining. One published report of expression on cell type, trying to replicate. May be antigen is not expressed rather than antibody poor as we have no real control.

There are no reviews that match your criteria.

Showing  1 - 3 的 3 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies