Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Virus

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Cell Selection, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 222328
View all Podocalyxin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
Ser23-Arg427
Accession # AAB61574

Specificity

Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Podocalyxin Antibody

Detection of Podocalyxin antibody in MDA-MB-231 Human Cell Line by Flow Cytometry.

Detection of Podocalyxin in MDA-MB-231 Human Cell Line by Flow Cytometry.

MDA-MB-231 (orange) or MG-63 (blue) human cell lines were stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histograms) or isotype control antibody (MAB003, open histograms), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

Podocalyxin antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

Podocalyxin in BG01V Human Stem Cells.

Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Podocalyxin in Human Kidney.

Podocalyxin was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomerular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Podocalyxin by Immunocytochemistry/ Immunofluorescence

Detection of Podocalyxin by Immunocytochemistry/ Immunofluorescence

Live-tracking of NPC rosette morphogenesis. (A–C) Live imaging of 1196a hiPSC expressing Lifeact-GFP (A) or PODXL-GFP (C) for 12 h after colonies were allowed to reattach for 2 h. (B) Confocal images of PODXL-GFP NPC rosettes stained with anti-PODXL. GFP localization mirrors endogenous PODXL localization. Note that, while a uniform prominent GFP signal is initially seen throughout the apical surface of the recently plated colony, at later time points, GFP-enriched regions are concentrated at the constricted centers of the rosettes (lumen). It is likely that, as the colony expands rapidly, the apical surface area of non-rosette cells dramatically increases, resulting in reduced GFP signal intensity per unit area. Scales as indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33178701), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Podocalyxin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/mL
Sample: MDA-MB-231 Human Cell Line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Immunohistochemistry

0.05-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

1 µg/mL
Sample: Recombinant Human Podocalyxin

Reviewed Applications

Read 7 reviews rated 4.4 using MAB1658 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

References

  1. Li, J. et al. (2001) DNA Seq. 12:407.
  2. Hara, T. et al. (1999) Immunity 11:567.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Gene Symbol

PODXL

UniProt

AAB61574

Additional Podocalyxin Products

Product Documents for Human Podocalyxin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Podocalyxin Antibody

For research use only

Citations for Human Podocalyxin Antibody

Customer Reviews for Human Podocalyxin Antibody (7)

4.4 out of 5
7 Customer Ratings
5 Stars
57%
4 Stars
29%
3 Stars
14%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 的 7 reviews Showing All
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  • Human Podocalyxin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Human
    Verified Customer | Posted 09/06/2021
    Human Podocalyxin Antibody MAB1658
  • Human Podocalyxin Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Podocalyxin Antibody MAB1658
  • Human Podocalyxin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: MAN13 hESC derived kidney organoid section
    Species: Human
    Verified Customer | Posted 12/01/2020
    Used at 1:200 dilution
    Human Podocalyxin Antibody MAB1658
  • Human Podocalyxin Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/20/2018
  • Human Podocalyxin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/08/2018
  • Human Podocalyxin Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human Podocalyxin Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: lysates from melanoma cells
    Species: Human
    Verified Customer | Posted 08/21/2017
    I used lysates from melanoma cells, melanoma extracellular vesicles (EV) and lysates from melanoma exosomes.
    The monoclonal mouse antibody, MAB 1658 yields a single sharp peak at 56 kda, corresponding to the expected size for PODXL. The signal is medium strong, as evident from the y-axis. The signal is identical in cell and EV lysates, but absent in the exosomes (this exo lysate has produced results with other antibodies). I used a regular 12-230 kDa matrix and standard settings. Both antibodies were used at a dilution of 1:50. The lysates were at a concentration of 0,6 ug/ul
    Human Podocalyxin Antibody MAB1658

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Protocols

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