Human ZnT-8 Antibody (815039)

R&D Systems | Catalog # MAB7936

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 815039
View all ZnT-8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZnT‑8
Lys268-Pro359
Accession # Q8IWU4

Specificity

Detects human ZnT‑8 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ZnT-8 Antibody (815039)

ZnT-8 antibody in Human Pancreas by Immunohistochemistry (IHC-P).

ZnT‑8 in Human Pancreas.

ZnT-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ZnT-8 Monoclonal Antibody (Catalog # MAB7936) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of ZNT-8 antibody in PANC-1 Human Cell Line antibody by Flow Cytometry.

Detection of ZNT-8 in PANC-1 Human Cell Line by Flow Cytometry.

PANC-1 human cell line was stained with Mouse Anti-Human ZNT-8 Monoclonal Antibody (Catalog # MAB7936, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human ZnT-8 Antibody (815039)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: PANC-1 human cell line fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012)

Reviewed Applications

Read 3 reviews rated 4.3 using MAB7936 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZnT-8

ZnT-8 (Zinc Transporter 8; also SLC30A8) is a 35-40 kDa member of the SLC (solute carrier)-30A subfamily, CDF family of proteins. It is expressed by pancreatic beta ‑cells and alpha -cells, B cells, and adipocytes and is known to play a role in Zn transport. In particular, ZnT-8 appears to transport zinc from the cytosol into secretory vesicles which, in the case of beta -cells, provides a necessary component for proper insulin processing and granule storage. Furthermore, it appears to facilitate glucose‑mediated insulin release. Human ZnT-8 is a multipass transmembrane (TM) protein that is 369 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-79) followed by six TM segments (aa 80-266) and a 103 aa C-terminal cytoplasmic tail. There is a key His-rich motif in the second cytoplasmic loop (aa 197‑205). ZnT-8 forms homodimers and possibly oligomers. There is one alternative start site at Met50. Over aa 268-359, human ZnT-8 shares 79% aa sequence identity with mouse ZnT-8.

Long Name

Zinc Transporter 8

Alternate Names

SLC30A8, ZnT8

Entrez Gene IDs

169026 (Human); 239436 (Mouse); 299903 (Rat)

Gene Symbol

SLC30A8

UniProt

Q8IWU4 (Human)

Additional ZnT-8 Products

Product Documents for Human ZnT-8 Antibody (815039)

Certificate of Analysis

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Product Specific Notices for Human ZnT-8 Antibody (815039)

For research use only

Related Research Areas

Diabetes
Glucose Homeostasis

Customer Reviews for Human ZnT-8 Antibody (815039) (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
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1 Stars
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Customer Images

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  • Human ZnT-8 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human ZnT-8 Antibody (815039) MAB7936
  • Human ZnT-8 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/07/2018
  • Human ZnT-8 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 08/31/2018
    Human ZnT-8 Antibody (815039) MAB7936

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Protocols

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