Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ki67/MKI67 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Mouse KI67/MKI67, between aminoacids 2000-2050 (2021-2036) Uniprot# E9PVX6.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31286466).

Localization

Nuclear

Marker

Proliferation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

351 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Ki67/MKI67 Antibody - BSA Free (NB110-89719) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Ki67/MKI67 Antibody: Cited in 27 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Ki67/MKI67 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-89719] - Ki67 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti-KI67 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This antibody generated a specific nuclear staining in epithelial cells and the staining was more intense in the cells close to the bases of crypts. Weak to moderate positivity was found at the cytoplasmic level also.
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NB110-89719-img0010.jpg
Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Detection of Ki67 in formalin-fixed paraffin embedded mouse intestine using NB110-89719.
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Ki-67 (NB110-89719) used at a dilution of 1:250.
Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Immunohistochemical analysis of mouse spleen.
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - IHC analysis of Ki67 in mouse cornea. Image courtesy of product review by Bo-Yie Chen.
Ki67/MKI67 Antibody - BSA Free

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719] -

Effect of saireito on the suppression of cell proliferation induced by 5-fluorouracil (5-FU) in mouse small intestines.5-FU (50 mg/kg) was injected i.p. while saireito (1000 mg/kg) was administered p.o. twice, 30 min before and 8 h after 5-FU injection. The jejunum was excised, sectioned, and Ki67 immunostaining was performed (A). The number of proliferative cells was counted (B). Data are presented as the mean ± SEM of 6 mice. #P < 0.05, versus normal (5-FU-untreated).
Ki67/MKI67 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-89719] -

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-89719] - The increase in iCM number with SB treatment is not due to increased transgene expression or changes in cell proliferation.Evaluation of gene expression of the transgenes at Day 2 via qPCR is not significantly different between +DMSO (black) control & +SB treatment groups (white, A). Evaluation of overall cell proliferation with Ki67 staining in MEFs with time for +DMSO (black) & +SB treatment groups (grey) (B). A time course evaluation of Troponin T (green) & Ki67 (red) did any dual labeled cells for either the +DMSO (top row) & +SB (bottom row) treated groups (C). Scale bar is 100 µM. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0089678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Ki67/MKI67 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 25733567)

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

reported in scientific literature (PMID 23419702)

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

reported in scientific literature (PMID 23643677)

Reviewed Applications

Read 3 reviews rated 5 using NB110-89719 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ki67/MKI67

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126

Entrez Gene IDs

4288 (Human); 17345 (Mouse)

Gene Symbol

MKI67

UniProt

P46013 (Human)

Additional Ki67/MKI67 Products

Product Documents for Ki67/MKI67 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ki67/MKI67 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Cell Cycle

Citations for Ki67/MKI67 Antibody - BSA Free

Customer Reviews for Ki67/MKI67 Antibody - BSA Free (3)

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Customer Images

Showing  1 - 3 的 3 reviews Showing All
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  • Ki-67/MKI67 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Skeletal muscle tissue
    Species: Mouse
    Verified Customer | Posted 11/30/2016
    Mouse skeletal muscle tissue stained for Ki67 (green), localized in nuclei. Tissue was co-stained for another muscle marker in red.
    Heat mediated low pH antigen retrieval was performed on sections prior to use. Permeabilization with 0.1% triton. The antibody was diluted 1/200. An Alexa Fluor anti-rabbit 488 was used to detect Ki67 antibody.
    Ki67/MKI67 Antibody - BSA Free NB110-89719
  • Name: James Xiao
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse lung tissue that was exposed to cigarette smoke
    Species: Mouse
    Verified Customer | Posted 04/07/2016
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse cornea
    Species: Mouse
    Verified Customer | Posted 02/06/2012
    Ki67/MKI67 Antibody - BSA Free NB110-89719

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Protocols

View specific protocols for Ki67/MKI67 Antibody - BSA Free (NB110-89719):


Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.


Antigen Unmasking
Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes.
Wash sections in dH2O three times for 5 minutes each.
Wash section in wash buffer for 5 minutes.
Block each section with 100-400 ul blocking solution (1X PBST, 5% goat serum) for 1 hour at room temperature.
Remove blocking solution and add 100-400 ul primary antibody diluted in 1X PBST, 5% goat serum to each section. Incubate overnight at 4C.
Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
Add 100-400 ul biotinylated secondary antibody, diluted in 1X PBST, 5% goat serum. Incubate 30 minutes at room temperature.
Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
Add 100-400 ul Striptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
Wash sections three times in wash buffer for 5 minutes each.
Add 100-400 ul DAB substrate to each section and monitor staining closely.
As soon as the sections develop, immerse slides in dH2O.
Wash sections in dH2O two times for 5 minutes each.
Mount coverslips.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Ki67/MKI67 Antibody - BSA Free

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    • A: This antibody is polyclonal, and thus it does not have a clone number.

    • A: The concentration is lot specific available upon request.

    • A:  Optimal concentrations and conditions for each application should be determined by the user.
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