MEK2 Antibody (OTI8G6)

Name: MEK2 Antibody (OTI8G6)
Brand: Novus Biologicals
SKU: NBP1-47836
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-47836

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI8G6

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Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K2 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

44.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK2 Antibody (OTI8G6)

Western Blot: MEK2 Antibody (OTI8G6) [NBP1-47836]

Western Blot: MEK2 Antibody (8G6) [NBP1-47836] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK2.

Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]

Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Human lung tissue using anti-MEK2 mouse monoclonal antibody.

Flow Cytometry: MEK2 Antibody (OTI8G6) [NBP1-47836]

Flow Cytometry: MEK2 Antibody (8G6) [NBP1-47836] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]

Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MEK2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]

Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MEK2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]

Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MEK2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]

Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Human endometrium tissue using anti-MEK2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK2 Antibody (OTI8G6) [NBP1-47836]

Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Staining of paraffin-embedded Human Kidney tissue using anti-MEK2 mouse monoclonal antibody.

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Applications for MEK2 Antibody (OTI8G6)

Application

Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK2

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases.Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.

Long Name

Mitogen-activated Protein Kinase Kinase 2

Alternate Names

MAP2K2, MKK2

Entrez Gene IDs

5605 (Human)

Gene Symbol

MAP2K2

Additional MEK2 Products

Product Documents for MEK2 Antibody (OTI8G6)

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Product Specific Notices for MEK2 Antibody (OTI8G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.