Mouse CXCR4 Antibody

Name: Mouse CXCR4 Antibody
Brand: R&D Systems
SKU: MAB21651R
Price: 499 USD
Availability: InStock

R&D Systems | Catalog # MAB21651R

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Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG_2B Clone # 247506R

View all CXCR4 Primary Antibodies »

Product Specifications

Immunogen

Y3 rat myeloid cell line transfected with mouse CXCR4
Met1-Ser359
Accession # P70658

Specificity

Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse CXCR4 Antibody

Detection of CXCR4 in HEK293 Human Cell Line Transfected with Mouse CXCR4 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse CXCR4 and eGFP was stained with (A) Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) or (B) Rat IgG2B isotype control antibody (Catalog # MAB0061) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry.

Mouse thymocytes were stained with Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R, filled histogram) or Rat IgG2B isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

CXCR4 in Mouse Splenocytes.

CXCR4 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CXCR4 in Mouse Spleen.

CXCR4 was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

CXCR4 in Mouse Testis.

CXCR4 was detected in perfusion fixed frozen sections of mouse testis using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in sperm cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Applications for Mouse CXCR4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse thymocytes and HEK293 Human Cell Line Transfected with Mouse CXCR4 and eGFP

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunohistochemistry

5-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spleen and perfusion fixed frozen sections of mouse testis

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).