Integrin beta 1/CD29 Antibody

Detection of Integrin beta 1/CD29 by Western Blot

Reduced activity of B4GALNT3 delta PA14 for O-GalNAc glycan and glycoprotein.A, B4GALNT3 WT or delta PA14 mutant was expressed in COS7 cells, immunoprecipitated, and blotted with anti-B4GALNT3 (left). The immunoprecipitated enzymes were incubated with GlcNAc beta 1-3GalNAc-pNP, and the reaction mixture was analyzed by reverse-phase HPLC. B, COS7 cells were cotransfected with the expression plasmids for transferrin-mycHis and B4GALNT3 WT or delta PA14. The cell lysates were subjected to Western blotting for B4GALNT3 and GAPDH (left). Secreted transferrin in the culture medium was purified using Ni2+ beads, and the proteins bound to the beads were blotted with WFA and anti-myc (right). C, Neuro2A cells were transfected with the expression plasmid for B4GALNT3 WT or delta PA14 or an empty vector. The lysates (input) were subjected to immunoprecipitation with anti-NCAM1 or anti-integrin-beta 1. Proteins bound to the beads were blotted with anti-NCAM1, anti-integrin-beta 1, and WFA. WFA, Wisteria floribunda agglutinin. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S0021925824019513), licensed under a CC-BY license. Not internally tested by R&D Systems.