Siglec-2/CD22 Antibody (2H1C4) - BSA Free
Novus Biologicals | Catalog # NBP2-37327
![Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]](https://beta-resources.rndsystems.com/images/products/Siglec-2-CD22-Antibody-2H1C4-Western-Blot-NBP2-37327-img0004.jpg "Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2H1C4
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human Siglec-2/CD22 (AA: 621-725) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
95.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse Siglec-2/CD22 Antibody (2H1C4) - BSA Free (NBP2-37327) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Siglec-2/CD22 Antibody (2H1C4) - BSA Free
Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]
Western Blot: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A432 (6) and NIH/3T3 (7) cell lysate.![Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]](https://beta-resources.rndsystems.com/images/products/Siglec-2-CD22-Antibody-2H1C4-Immunocytochemistry-Immunofluorescence-NBP2-37327-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]")
Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]
Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]](https://beta-resources.rndsystems.com/images/products/Siglec-2-CD22-Antibody-2H1C4-Immunohistochemistry-NBP2-37327-img0008.jpg "Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]")
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis of cerebellum tissues using CD22 mouse mAb with DAB staining.![Flow Cytometry: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]](https://beta-resources.rndsystems.com/images/products/Siglec-2-CD22-Antibody-2H1C4-Flow-Cytometry-NBP2-37327-img0006.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]")
Flow Cytometry: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]
Flow Cytometry: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).![Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]](https://beta-resources.rndsystems.com/images/products/Siglec-2-CD22-Antibody-2H1C4-Western-Blot-NBP2-37327-img0002.jpg "Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]")
Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]
Western Blot: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis using CD22 mAb against human CD22 recombinant protein. (Expected MW is 37 kDa).![Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]](https://beta-resources.rndsystems.com/images/products/Siglec-2-CD22-Antibody-2H1C4-Western-Blot-NBP2-37327-img0003.jpg "Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]")
Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]
Western Blot: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]](https://beta-resources.rndsystems.com/images/products/Siglec-2-CD22-Antibody-2H1C4-Immunohistochemistry-NBP2-37327-img0007.jpg "Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]")
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis of cervical cancer tissues using CD22 mouse mAb with DAB staining.![ELISA: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]](https://beta-resources.rndsystems.com/images/products/Siglec-2-CD22-Antibody-2H1C4-ELISA-NBP2-37327-img0001.jpg "ELISA: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]")
ELISA: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]
ELISA: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Applications for Siglec-2/CD22 Antibody (2H1C4) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Siglec-2/CD22
Long Name
Sialic Acid Binding Ig-like Lectin 2
Alternate Names
BL-CAM, CD22, Siglec2
Gene Symbol
CD22
Additional Siglec-2/CD22 Products
Product Documents for Siglec-2/CD22 Antibody (2H1C4) - BSA Free
Product Specific Notices for Siglec-2/CD22 Antibody (2H1C4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Siglec-2/CD22 Antibody (2H1C4) - BSA Free
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Q: I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?
A: We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.
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Associated Pathways
