A-RAF Antibody (OTI2G9) - Azide and BSA Free

Name: A-RAF Antibody (OTI2G9) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-70201
Price: 599 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-70201

Submit a review

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # OTI2G9

Format

Azide and BSA Free

View all A-Raf Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 145-365 of human ARAF (NP_001645) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for A-RAF Antibody (OTI2G9) - Azide and BSA Free

Western Blot: A-RAF Antibody (OTI2G9)Azide and BSA Free [NBP2-70201]

Western Blot: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF.

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]

Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

View 11 more images

Applications for A-RAF Antibody (OTI2G9) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: A-Raf

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Long Name

v-Raf Murine Sarcoma 3611 Viral Oncogene Homolog

Alternate Names

ARaf, PKS, PKS2, RAFA1

Gene Symbol

ARAF

Additional A-Raf Products

Product Documents for A-RAF Antibody (OTI2G9) - Azide and BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for A-RAF Antibody (OTI2G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.