ABCG2/CD338 Antibody (3G8) - BSA Free

Novus Biologicals | Catalog # NBP2-22124

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G8

Format

BSA Free
View all ABCG2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ABCG2 expressed in E. coli. [Uniprot: Q9UNQ0]

Marker

Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60-70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABCG2/CD338 Antibody (3G8) - BSA Free

Western Blot: ABCG2/CD338 Antibody (3G8)BSA Free [NBP2-22124]

Western Blot: ABCG2/CD338 Antibody (3G8)BSA Free [NBP2-22124]

Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.
Immunocytochemistry/ Immunofluorescence: ABCG2/CD338 Antibody (3G8) - BSA Free [NBP2-22124]

Immunocytochemistry/ Immunofluorescence: ABCG2/CD338 Antibody (3G8) - BSA Free [NBP2-22124]

Immunocytochemistry/Immunofluorescence: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
Immunohistochemistry-Paraffin: ABCG2/CD338 Antibody (3G8) - BSA Free [NBP2-22124]

Immunohistochemistry-Paraffin: ABCG2/CD338 Antibody (3G8) - BSA Free [NBP2-22124]

Immunohistochemistry-Paraffin: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.
Flow Cytometry: ABCG2/CD338 Antibody (3G8) - BSA Free [NBP2-22124]

Flow Cytometry: ABCG2/CD338 Antibody (3G8) - BSA Free [NBP2-22124]

Flow Cytometry: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of HepG2 cells using ABCG2 mouse mAb (green) and negative control (purple).
ELISA: ABCG2/CD338 Antibody (3G8) - BSA Free [NBP2-22124]

ELISA: ABCG2/CD338 Antibody (3G8) - BSA Free [NBP2-22124]

ELISA: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).
ABCG2/CD338 Antibody (3G8) - BSA Free

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal ABCG2/CD338 Antibody (3G8) [NBP2-22124]

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Human brain microvascular endothelial cells stained for cilia (red) and ABCG2 (green) and nuclear DAPI (blue). Image from a verified customer review.
ABCG2/CD338 Antibody (3G8) - BSA Free

Western Blot: ABCG2/CD338 Antibody (3G8) - BSA Free [NBP2-22124] -

(A–D) Gene expression of drug transporters in breast cancer patients (A) ABCC1, (B) ABCG2 in local cohort (C) ABCC1, (D) ABCG2 in TCGA cohort and (E–G) Protein expression of drug transporters (E) Representative blots in adjacent normal (N) and tumor (T) tissues, (F) Densitometric analysis of ABCG2 and (G) ABCC1 levels in adjacent normal and tumor tissues. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36309544), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ABCG2/CD338 Antibody (3G8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
In Western blot, a dimer can be seen at 60-70 kDa representing ABCG2.

Reviewed Applications

Read 2 reviews rated 3 using NBP2-22124 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABCG2

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.

Long Name

ATP-binding Cassette Transporter G2

Alternate Names

ABCP, BCRP, CD338, MXR

Entrez Gene IDs

9429 (Human)

Gene Symbol

ABCG2

UniProt

Q9UNQ0 (Human)

Additional ABCG2 Products

Product Documents for ABCG2/CD338 Antibody (3G8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ABCG2/CD338 Antibody (3G8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ABCG2/CD338 Antibody (3G8) - BSA Free

Customer Reviews for ABCG2/CD338 Antibody (3G8) - BSA Free (2)

3 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
50%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • ABCG2/CD338 Antibody (3G8) - BSA Free
    Name: KARTHIKEYAN THIRUGNANAM
    Application: Immunocytochemistry
    Sample Tested: human brain microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 12/20/2024
    Human brain microvascular endothelial cells stained for cilia (red) and ABCG2 (green) and nuclear DAPI (blue).
    ABCG2/CD338 Antibody (3G8) - BSA Free NBP2-22124
  • ABCG2/CD338 Antibody (3G8) - BSA Free
    Name: Pauline Jeannot
    Application: Immunocytochemistry
    Sample Tested: A2058 human melanoma cell line
    Species: Human
    Verified Customer | Posted 11/16/2023
    A2058 melanoma cell line stained with NBP2-22124 dilution 1:100. No signal
    ABCG2/CD338 Antibody (3G8) - BSA Free NBP2-22124
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

View specific protocols for ABCG2/CD338 Antibody (3G8) - BSA Free (NBP2-22124):

ABCG2/CD338 Antibody (3G8):
Flow Cytometry Protocol

Solutions and Reagents:

1X PBS
Blocking buffer: 0.5% BSA in 1X PBS
Filter buffer: 0.1%Triton-X100 and Blocking buffer
Ice cold 4% paraformaldehyde (1% solution -optional for storing samples)
Fluorescently-conjugated secondary antibody (various forms)

Protocol

2.1. Collect 1x10^6 cells/sample.
2.2. Wash cells once with blocking buffer.
2.3. Fix cells with 4% paraformaldehyde and incubate at 4C for 30 min.
2.4 Permeabilize cells: Add 0.5 ml filter buffer(0.1%Triton-X100 and Blocking buffer) and incubate at 0 degree C for 15 min.
2.4. Wash cells once with blocking buffer.
2.5. Add 0.5 ml filter buffer and incubate at 0C for 15 min.
2.6. Wash cells twice with blocking buffer.
2.7. Incubate cells in blocking buffer for 10 min at room temperature.
2.8. Add primary antibody at the appropriate dilution and incubate for 30 min at room temperature.
2.9. Wash twice with blocking buffer and incubate with fluorescently-conjugated secondary antibody for 30 min at room temperature.
2.10. Wash cells twice with blocking buffer.
2.11. Re-suspend cells in 1X PBS and analyze on flow cytometry. Samples can be kept in 1% paraformaldehyde at 4C overnight.

Advice: Keep the cells in the dark on ice or at 4C in a fridge until your scheduled time for analysis. Analyze the cells on the flow cytometer as soon as possible.



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