Acetylcholinesterase/ACHE Antibody

Novus Biologicals | Catalog # NB100-1519

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry Free-Floating, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Acetylcholinesterase/ACHE Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence QFDHYSKQDRCSDL corresponding to C-Terminus according to NP_000656.1.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Marker

Early Neuronal Development Marker

Specificity

This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat Acetylcholinesterase/ACHE Antibody (NB100-1519) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Acetylcholinesterase/ACHE Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Acetylcholinesterase/ACHE Antibody

Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519]

Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519]

Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Staining (0.3ug/ml) of Jurkat (A) and (0.5ug/ml) HepG2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519]

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519]

Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519]

Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519]

Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Acetylcholinesterase/ACHE Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
This Acetylcholinesterase/ACHE antibody is validated for IHC-FrFl from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-1519 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetylcholinesterase/ACHE

Acetylcholinesterase, or ACHE, hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Alternate Names

ACHE, ARACHE, N-ACHE

Entrez Gene IDs

43 (Human); 11423 (Mouse)

Gene Symbol

ACHE

UniProt

NP_000656. (Human)

Additional Acetylcholinesterase/ACHE Products

Product Documents for Acetylcholinesterase/ACHE Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Acetylcholinesterase/ACHE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Acetylcholinesterase/ACHE Antibody

Customer Reviews for Acetylcholinesterase/ACHE Antibody (1)

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Customer Images

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  • Acetylcholinesterase/ACHE Antibody
    Name: Anonymous
    Application: IHC-Floating section
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 11/21/2016
    Positive ACHE staining (green) in neurons of the mouse brain. Fixed in 4% paraformaldehyde, and sectioned at 50um using a vibratome
    Floating sections were incubated overnight at 4C with a 1:200 dilution of the above ACHE antibody, washed and then incubated for 1 hour at room temperature with a 1:500 dilution of donkey anti-rabbit DyLight 594 secondary antibody (Cat#NBP1-75637).
    Acetylcholinesterase/ACHE Antibody NB100-1519

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Protocols

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