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alpha-Smooth Muscle Actin Antibody

Novus Biologicals | Catalog # NB300-978

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rabbit

Cited:

Human, Mouse, Rat, Rabbit

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all alpha-Smooth Muscle Actin Primary Antibodies »

Product Specifications

Immunogen

Alpha-Smooth Muscle Actin Antibody is made to a peptide with sequence EEEDSTALVC corresponding to N-Terminus according to NP_001604.1, NP_001135417.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25619662). Rabbit reactivity reported in scientific literature (PMID: 30480706 and PMID: 33263285).

Marker

Mesenchymal Cell Marker

Specificity

Variants NP_001604.1 and NP_001135417.1 encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody

Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978]

Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978]

Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] - (1ug/ml) staining of Mouse Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody [NB300-978]

Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody [NB300-978]

Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody [NB300-978] - SMA antibody staining on mouse fibroblasts. Primary antibody at 1:1000, incubation at 4C overnight. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NB300-978]

Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NB300-978]

Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NB300-978] - Infarcted mouse heart frozen tissue section immunostained with alpha-Smooth Muscle Actin Antibody. IHC-Fr image submitted by a verified customer review.
alpha-Smooth Muscle Actin Antibody [NB300-978] - (1ug/ml) staining of HeLa (A) and (2ug/ml) NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
alpha-Smooth Muscle Actin Antibody

Immunohistochemistry-Paraffin: Goat Polyclonal alpha-Smooth Muscle Actin Antibody [NB300-978]

Immunohistochemistry-Paraffin: Goat Polyclonal alpha-Smooth Muscle Actin Antibody [NB300-978] - Analysis of alpha-Smooth Muscle Actin Antibody on mouse heart tissue. Image from a verified customer review.
alpha-Smooth Muscle Actin Antibody

Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] -

Effect of Nebivolol on TGF-beta 1, p-Smad2/3, and alpha -SMA proteins expression. (A) representative western blot membranes of TGF-beta 1, Smad2/3, p-Smad2/3, alpha -SMA, and beta -actin proteins for all studied groups. (B–D) Expressions of TGF-beta 1, p-Smad2/3/t-Smad2/3, and alpha -SMA proteins were represented densitometrically using bands in (A). Bars are represented as mean +/- SEM. The Tukey-Kramar test was used to analyze the significant differences between groups after conducting a one-way ANOVA test, where ###p < 0.001, compared to the sham group, *p < 0.05,**p < 0.01, and ***p < 0.001, compared to CLP group, $$p < 0.01, and $$$p < 0.001, compared to CLP + Neb4 group. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-64577-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
alpha-Smooth Muscle Actin Antibody

Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] -

Activation of multiple signaling pathways in TEC challenged by cytokines. Primary human renal TECs were treated with the cytokine cocktail. (A, B) Western blot of PI3K–Akt pathway, NFkb pathway, and Erk signaling pathway in cells treated with cytokine cocktail. (C) Densitometric quantitation of AktS473, pNFkB65, ErkTh202/Ty204, and pTenS380. All Western blots are representative of two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
alpha-Smooth Muscle Actin Antibody

Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] -

Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma, 6nM TNF alpha, and 3nM IL1 beta ). (A, B) Expression of Snail, E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. (C, D) Densitometric quantitation of protein in (A, B). All Western blots are representative of at least three independent experiments. *P < 0.05, ***P < 0.0001 versus to cells without treatment, analysis is multiple t-tests, pairwise comparison of individual time-point to control indicated same p-value. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
alpha-Smooth Muscle Actin Antibody

Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] -

Activation of multiple signaling pathways in TEC challenged by cytokines. Primary human renal TECs were treated with the cytokine cocktail. (A, B) Western blot of PI3K–Akt pathway, NFkb pathway, and Erk signaling pathway in cells treated with cytokine cocktail. (C) Densitometric quantitation of AktS473, pNFkB65, ErkTh202/Ty204, and pTenS380. All Western blots are representative of two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for alpha-Smooth Muscle Actin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 2 ug/mL
Application Notes
Approx 45 kDa band observed in HeLa, NIH3T3, Mouse Duodenum and Human Heart lyates, and also in preliminary testing of Rat Duodenum lysates (calculated MW of 42 kDa according to NP_001604 and NP_031418.1). Alpha-Smooth Muscle Actin Antibody is validated for IHC-Fr, ICC/IF from verified customer reviews.

Reviewed Applications

Read 8 reviews rated 5 using NB300-978 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2.El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G.,... Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M.,... Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Entrez Gene IDs

59 (Human)

Gene Symbol

ACTA2

UniProt

P62736 (Human)

Additional alpha-Smooth Muscle Actin Products

Product Documents for alpha-Smooth Muscle Actin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for alpha-Smooth Muscle Actin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Smooth Muscle Actin Antibody

Customer Reviews for alpha-Smooth Muscle Actin Antibody (8)

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Customer Images

Showing  1 - 5 of 8 reviews Showing All
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  • alpha-Smooth Muscle Actin Antibody
    Name: KARTHIKEYAN THIRUGNANAM
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse heart
    Species: Mouse
    Verified Customer | Posted 12/20/2024
    Mouse heart IHC
    alpha-Smooth Muscle Actin Antibody NB300-978
  • alpha-Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Mouse
    Verified Customer | Posted 09/15/2021
    SMA antibody staining on mouse fibroblasts.
    Primary antibody 1:1000 concentration, incubation overnight at 4C.
    alpha-Smooth Muscle Actin Antibody NB300-978
  • alpha-Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 03/31/2021
    left: control fibroblasts Right: SMA overexpression
    alpha-Smooth Muscle Actin Antibody NB300-978
  • alpha-Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Adult testis
    Species: Mouse
    Verified Customer | Posted 01/14/2021
    Green channel is sma, colocalize with CD34(red).
    primary antibody 1:200 dilution.
    alpha-Smooth Muscle Actin Antibody NB300-978
  • alpha-Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse heart frozen sections
    Species: Mouse
    Verified Customer | Posted 01/30/2020
    Infarcted mouse heart immunostained with SMA antibody
    Mouse frozen heart after myocardial infarction PFA fixed Primary antibody 1:200 Secondary antibody 1:500
    alpha-Smooth Muscle Actin Antibody NB300-978
  • alpha-Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 10/09/2019
    Green color is SMA by using NB300-978 antibody in fibroblast tissue.
    alpha-Smooth Muscle Actin Antibody NB300-978
  • alpha-Smooth Muscle Actin Antibody
    Name: Paramananda Saikia
    Application: Immunohistochemistry-Frozen
    Sample Tested: corneal stromal cells
    Species: Rabbit
    Verified Customer | Posted 10/23/2018
  • alpha-Smooth Muscle Actin Antibody
    Name: Paramananda Saikia
    Application: Immunocytochemistry
    Sample Tested: Eye lens tissue
    Species: Rabbit
    Verified Customer | Posted 05/31/2018

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for alpha-Smooth Muscle Actin Antibody

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  • Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?

    A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

  • Q: Which antibodies would you recommend for the best performance in IHC?

    A: NB300-978, NBP1-30894, NBP2-22120, NBP2-32808, NBP2-33006, and NBP2-34522 have the broadest IHC applications, as they additionally list IHC-Paraffin and IHC-Frozen. To narrow the list down further, we would recommend using either NB300-978 or NBP2-33006 since these products have the most ratings and cited publications for use in IHC methods. NB300-978 has IHC-Free-Floating listed as an application as well.

  • Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?

    A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

  • Q: Which antibodies would you recommend for the best performance in IHC?

    A: NB300-978, NBP1-30894, NBP2-22120, NBP2-32808, NBP2-33006, and NBP2-34522 have the broadest IHC applications, as they additionally list IHC-Paraffin and IHC-Frozen. To narrow the list down further, we would recommend using either NB300-978 or NBP2-33006 since these products have the most ratings and cited publications for use in IHC methods. NB300-978 has IHC-Free-Floating listed as an application as well.

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