AMPK alpha 1 Antibody (2B7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80575

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B7

Format

Azide and BSA Free
View all AMPK alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AMPK alpha 1 Antibody (2B7) - Azide and BSA Free

Western Blot: AMPK alpha 1 Antibody (2B7)Azide and BSA Free [NBP2-80575]

Western Blot: AMPK alpha 1 Antibody (2B7)Azide and BSA Free [NBP2-80575]

Western Blot: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.
Immunohistochemistry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

Immunohistochemistry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

Immunohistochemistry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining. Image from the standard format of this antibody.
Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Flow cytometric analysis of PC-2 cells using AMPK alpha 1 mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.
Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.
ELISA: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

ELISA: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]

ELISA: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng). Image from the standard format of this antibody.
Simple Western: AMPK alpha 1 Antibody (2B7)Azide and BSA Free [NBP2-80575]

Simple Western: AMPK alpha 1 Antibody (2B7)Azide and BSA Free [NBP2-80575]

Simple Western: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for AMPK alpha 1 Antibody (2B7) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Simple Western

1:50

Western Blot

1:500
Application Notes
This AMPK alpha 1 (2B7) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:50. Separated by Size-Wes, Sally Sue/Peggy Sue.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK alpha 1

AMPK alpha 1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increases the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Gene Symbol

PRKAA1

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 Antibody (2B7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AMPK alpha 1 Antibody (2B7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

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Protocols

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FAQs

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