Androgen R/NR3C4 Antibody

Novus Biologicals | Catalog # NB100-1446

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Equine, Feline

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Androgen R/NR3C4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence EVQLGLGRVYPRPPSC corresponding to N-Terminus according to NP_000035.2.

Reactivity Notes

Feline and Equine reactivity reported from a verified customer review.

Localization

Nuclear

Specificity

This antibody is expected to recognize isoform 1 (NP_000035.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Androgen R/NR3C4 Antibody

Western Blot: Androgen R/NR3C4 Antibody [NB100-1446]

Western Blot: Androgen R/NR3C4 Antibody [NB100-1446]

Western Blot: Androgen R/NR3C4 Antibody [NB100-1446] - Staining of Human Cerebellum (A) and (0.1ug/ml) negative control Pancreas (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446]

Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446]

Immunocytochemistry/Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody [NB100-1446]

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody [NB100-1446]

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody [NB100-1446] - (2ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Chromatin Immunoprecipitation: Androgen R/NR3C4 Antibody [NB100-1446] - (Protein G) measuring FKBP5 enrichment.
Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446]

Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446]

Immunocytochemistry/Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Androgen R/NR3C4 Antibody

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] -

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in equine epididymis. NB100-1446 was diluted to 1 ug/mL and left on tissue sections for 30 min at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval using an EDTA based solution. Image from a verified customer review.
Androgen R/NR3C4 Antibody

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] -

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in canine epididymis. NB100-1446 was diluted to 1 ug/mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval in an EDTA based buffer. Image from a verified customer review.
Androgen R/NR3C4 Antibody

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] -

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in feline epididymis. NB100-1446 was diluted to 1 ug/mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval in an EDTA based buffer. Image from a verified customer review.
Androgen R/NR3C4 Antibody

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] -

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in mouse testis. NB100-1446 was diluted to 1 ug/mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval in an EDTA based buffer. Image from a verified customer review.
Androgen R/NR3C4 Antibody

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] -

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in human testis. NB100-1446 was diluted to 1ug per mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat-induced epitope retrieval in an EDTA-based solution. Image from a verified customer review.

Applications for Androgen R/NR3C4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Paraffin

2-3 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

1-0.5ug/ml
Application Notes
ChIP: A 6-fold increased presence of the FKBP5 locus observed when comparing the precipitations using Goat Anti-Androgen Receptor with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
WB: Approx. 95 kDa band observed in human brain and human heart lysates (calculated MW of 99 kDa band according to NP_000035).
IHC-P: Human prostate shows nuclear staining in the secretory cells of the gland.

Reviewed Applications

Read 5 reviews rated 4.8 using NB100-1446 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Androgen R/NR3C4

Like all steroid receptors, the androgen receptor has several functional domains. Androgen receptors have a DNA binding domain, a hinge section, and the hormone binding domain. There is only one active form of the androgen receptor. Smaller forms represent proteolytic fragments that are not considered physiologically relevant. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Entrez Gene IDs

367 (Human); 11835 (Mouse); 24208 (Rat)

Gene Symbol

AR

UniProt

P10275

Additional Androgen R/NR3C4 Products

Product Documents for Androgen R/NR3C4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Androgen R/NR3C4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Androgen R/NR3C4 Antibody

Customer Reviews for Androgen R/NR3C4 Antibody (5)

4.8 out of 5
5 Customer Ratings
5 Stars
80%
4 Stars
20%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Androgen R/NR3C4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Testis
    Species: Human
    Verified Customer | Posted 11/17/2023
    Androgen receptor immunoreactivity in human testis. NB100-1446 was diluted to 1ug per mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat-induced epitope retrieval in an EDTA-based solution.
    Androgen R/NR3C4 Antibody NB100-1446
  • Androgen R/NR3C4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Epididymis
    Species: Equine
    Verified Customer | Posted 10/27/2023
    Androgen receptor immunoreactivity in horse epididymis. NB100 1446 was diluted to 1ug per mL and left on tissue sections for 30min at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval using an EDTA based solution
    Androgen R/NR3C4 Antibody NB100-1446
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Androgen R/NR3C4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Epididymis
    Species: Canine
    Verified Customer | Posted 10/27/2023
    Androgen receptor immunoreactivity in dog epididymis. NB100 1446 was diluted to 1ug per mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval in an EDTA based buffer.
    Androgen R/NR3C4 Antibody NB100-1446
  • Androgen R/NR3C4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Epididymis
    Species: Feline
    Verified Customer | Posted 10/27/2023
    Androgen receptor immunoreactivity in cat epididymis. NB100 1446 was diluted to 1ug per mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval in an EDTA based buffer.
    Androgen R/NR3C4 Antibody NB100-1446
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Androgen R/NR3C4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Testis
    Species: Mouse
    Verified Customer | Posted 10/27/2023
    Androgen receptor immunoreactivity in mouse testis. NB100 1446 was diluted to 1ug per mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval in an EDTA based buffer
    Androgen R/NR3C4 Antibody NB100-1446

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail