Anti- Satumomab (Anti-Idiotype) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Satumomab by Western Blot. Western blot shows lysates of Daclizumab (negative control), Basiliximab (negative control), Satumomab, Teplizumab (negative control), and Alemtuzumab (negative control). PVDF membrane was probed with 2 µg/mL of Anti- Satumomab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Satumomab at approximately 250 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The anti-Satumomab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Satumomab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.
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