Anti- Teprotumumab (Anti-Idiotype) Antibody
R&D Systems | Catalog # MAB96341
Key Product Details
Species Reactivity
Multi-Species
Applications
Binding Inhibition, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 975547
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Product Specifications
Immunogen
Full-length Teprotumumab
Specificity
Detects Teprotumumab in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Anti- Teprotumumab (Anti-Idiotype) Antibody
Detection of Anti-IGFLR-1 (Teprotumumab) on Human MCF-7 cells by flow cytometry.
MCF-7 human cell line was stained with Human anti-Human IGFLR-1 (Teprotumumab) Monoclonal Antibody (Catalog # MAB9718) then Anti-Teprotumumab (Anti-Idiotype) (Catalog # MAB96341, filled histogram) or Mouse IgG2Aisotype control antibody(Catalog # MAB003, open histogram) followed by PE-conjugated anti-mouse IgG secondary antibody (Catalog # F0102B).
Anti-IGFLR-1 (Teprotumumab) binding to MCF-7 Human Cell Line is Blocked by Anti-Teprotumumab (Anti-Idiotype) Antibody.
In a functional flow cytometry test, Biotinylated Anti-IGFLR-1 (Teprotumumab) (Catalog # FAB9718B) binds to MCF-7 human cell line (black dotted line). Binding is completely blocked (orange histogram) by 0.25 µg/mL of Anti-Teprotumumab (Anti-idiotype) Monoclonal Antibody (Catalog# MAB96341), but not by Normal Mouse IgG2A(Catalog # MAB003) (blue line). Cells were stained with APC-conjugated Streptavidin (Catalog # F0050).Applications for Anti- Teprotumumab (Anti-Idiotype) Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: MCF-7 Human Cell line stained anti-IGFLR-1 (Teprotumumab ), Catalog # MAB9718
Sample: MCF-7 Human Cell line stained anti-IGFLR-1 (Teprotumumab ), Catalog # MAB9718
Binding Inhibition
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Teprotumumab
Additional Teprotumumab Products
Product Documents for Anti- Teprotumumab (Anti-Idiotype) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Anti- Teprotumumab (Anti-Idiotype) Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Anti- Teprotumumab (Anti-Idiotype) Antibody
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Q: What is an anti-idiotype antibody?
A: An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody. We have anti-idiotype antibodies to commecially available therapeutic antibodies or biosimilars.
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