Embryonic Stem Cell Antibody Panels
These antibody panels are designed for analyzing the differentiation status of human embryonic stem (ES) cells by evaluating ES cell marker expression. Each panel contains a group of antibodies that are recognized as markers for undifferentiated human ES cells. Panels can be applied to both flow cytometry- and immunohistochemistry-based applications.
While the undifferentiated/pluripotent state of ES cells can be best defined functionally, a number of molecular markers have been used to characterize it. Pluripotent ES cells can be characterized by high level expression of the transcription factors Oct-3/4 (also termed Oct-3 or Oct-4) and Nanog. A critical amount of Oct-3/4 and Nanog expression is required to sustain stem-cell pluripotency. When ES cells are induced to differentiate, Oct-3/4 and Nanog are down-regulated.
The undifferentiated state of ES cells is often characterized by the expression of the cell surface antigens, SSEA-1 and SSEA-4. Undifferentiated primate ES cells, human ES cells, and human Embryonic Carcinoma (EC) cells express SSEA-4, but not SSEA-1. In contrast, undifferentiated mouse ES cells do express SSEA-1 but do not express SSEA-4. Undifferentiated human EC, ES, and embryonic germ cells have also been shown to express a very high level of alkaline phosphatase. Expression levels of alkaline phosphatase decrease following stem cell differentiation.
Human Embryonic Stem Cell Marker Antibody Panel (Catalog # SC008)
Each Kit Contains the following antibodies:
- Anti-Alkaline Phosphatase
- Anti-Nanog
- Anti-Oct-3/4
- Anti-SSEA-1
- Anti-SSEA-4
Human Embryonic Stem Cell Marker Antibody Panel Plus (Catalog # SC009)
Each Kit Contains the following antibodies:
- Anti-CD9
- Anti-E-Cadherin
- Anti-Nanog
- Anti-Oct-3/4
- Anti-Podocalyxin
- Anti-SOX2
- Anti-SSEA-1
- Anti-SSEA-4
A  B  C  |
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| An Embryoid Body Derived from Human Embryonic Stem Cells Stained for Pluripotency Markers. Cells were stained using antibodies from the Human Embryonic Stem Cell Marker Antibody Panel (Catalog # SC008) for Nanog (red; A) and the nuclei counterstained with DAPI (blue; A) or for Oct-3/4 (green; B). Merge of images in (A) and (B) shows the overlap of the 3 fluorochromes (white; C).
Images courtesy of Dr. Ronald McKay, NINDS. |
Flow Cytometry analysis of SSEA-4 expression. Reactivity of human embryonic stem cells stained with Mouse Anti-SSEA-4 Monoclonal Antibody (Catalog # MAB1435) (green) or isotype control (black). Cells were stained using a PE-conjugated goat anti-mouse IgG secondary antibody. |