Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free

Novus Biologicals | Catalog # NBP2-52932

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0468

Format

BSA Free
View all Apolipoprotein A-IV/ApoA4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P06727, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free

Apolipoprotein A-IV/ApoA4 Antibody (CL0468)

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] - Analysis in human small intestine and pancreas tissues using NBP2-52932 antibody. Corresponding APOA4 RNA-seq data are presented for the same tissues.
Western Blot: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932]

Western Blot: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932]

Western Blot: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] - Lane 1: Marker [kDa], Lane 2: Human plasma
Apolipoprotein A-IV/ApoA4 Antibody (CL0468)

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Apolipoprotein A-IV/ApoA4 Antibody (CL0468)

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Apolipoprotein A-IV/ApoA4 Antibody (CL0468)

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -Staining of human colon shows moderate positivity in plasma in blood vessels.
Apolipoprotein A-IV/ApoA4 Antibody (CL0468)

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP2-52932 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein A-IV/ApoA4

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Alternate Names

Apolipoprotein AIV

Gene Symbol

APOA4

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free

Certificate of Analysis

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Product Specific Notices for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

Customer Reviews for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free (1)

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  • Apolipoprotein A-IV/ApoA4 Antibody (CL0468)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 01/05/2022
    Staining in colon tissue
    Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free NBP2-52932

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Protocols

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FAQs for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free

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  • Q: I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?

    A:

    All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

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