Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43969

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0468

Format

Azide and BSA Free
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Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P06727, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free

Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Analysis in human small intestine and pancreas tissues using NBP3-43969 antibody. Corresponding Apolipoprotein A-IV/ApoA4 RNA-seq data are presented for the same tissues.
Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free Western Blot: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Western Blot: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Lane 1: Marker [kDa]
Lane 2: Human plasma
Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Staining of human colon shows moderate positivity in plasma in blood vessels.
Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP3-43969]

Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Applications for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein A-IV/ApoA4

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Alternate Names

Apolipoprotein AIV

Gene Symbol

APOA4

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - Azide and BSA Free

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  • Q: I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?

    A:

    All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

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